For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Rabbit polyclonal to SGT1
HumanPredicted to work with:
Rabbit, Goat, Horse, Guinea pig, Dog, Pig, Rhesus monkey, Orangutan, Elephant
Synthetic peptide, corresponding to a region between residue 450 and 500 of human SGT1 (NP_009196.1)
- HeLa and 293T whole cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
||Use at 2-5 µg/mg of lysate.
Information by UniProt
- ecd antibody
- ECD_HUMAN antibody
- Ecdysoneless homolog antibody
Western blot - Anti-SGT1 antibody (ab99293)
All lanes : Anti-SGT1 antibody (ab99293) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 73 kDa
Exposure time: 3 seconds
Immunoprecipitation - Anti-SGT1 antibody (ab99293)
Lane 1 : Detection of Human SGT1 by Immunoprecipitation from Hela ( 1 mg for IP, 20% of IP loaded), using 3µg/mg lysate.
Lane 2 : Control IgG
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"