Overview

  • Product name

  • Description

    Rabbit polyclonal to SGT1/ECD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Goat, Horse, Guinea pig, Dog, Pig, Rhesus monkey, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human SGT1/ECD aa 450-500.
    Database link: NP_009196.1

  • Positive control

    • HeLa and 293T whole cell lysate
  • General notes

     This product was previously labelled as SGT1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab99293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

Images

  • All lanes : Anti-SGT1/ECD antibody (ab99293) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 73 kDa


    Exposure time: 3 seconds
  • Lane 1 : Detection of Human SGT1/ECD by Immunoprecipitation from Hela ( 1 mg for IP, 20% of IP loaded), using 3µg/mg lysate. Lane 2 : Control IgG

References

This product has been referenced in:

  • Xia HG  et al. Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death. J Cell Biol 210:705-16 (2015). Read more (PubMed: 26323688) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH-3T3)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
NIH-3T3
Blocking step
Milk as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Dec 17 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up