Key features and details
- Rabbit polyclonal to SGT1/ECD
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SGT1/ECD antibody
See all SGT1/ECD primary antibodies
DescriptionRabbit polyclonal to SGT1/ECD
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Goat, Horse, Guinea pig, Dog, Pig, Rhesus monkey, Orangutan, Elephant
Synthetic peptide corresponding to Human SGT1/ECD aa 450-500.
Database link: NP_009196.1
- HeLa and 293T whole cell lysate
This product was previously labelled as SGT1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 73 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
- Information by UniProt
- ecd antibody
- ECD_HUMAN antibody
- Ecdysoneless homolog antibody
All lanes : Anti-SGT1/ECD antibody (ab99293) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 73 kDa
Exposure time: 3 seconds
Lane 1 : Detection of Human SGT1/ECD by Immunoprecipitation from Hela ( 1 mg for IP, 20% of IP loaded), using 3µg/mg lysate. Lane 2 : Control IgG
ab99293 has been referenced in 1 publication.
- Xia HG et al. Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death. J Cell Biol 210:705-16 (2015). PubMed: 26323688