Anti-SH2B1/PSM antibody (ab196575)
Key features and details
- Rabbit polyclonal to SH2B1/PSM
- Suitable for: ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SH2B1/PSM antibody
See all SH2B1/PSM primary antibodies -
Description
Rabbit polyclonal to SH2B1/PSM -
Host species
Rabbit -
Specificity
ab196575 detects endogenous level of total SH2B1/PSM protein.
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Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment corresponding to Human SH2B1/PSM (internal sequence).
Database link: Q9NRF2 -
General notes
This product was previously labelled as SH2B1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS is without Mg2+ and Ca2+. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab196575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/200. |
Target
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Function
Adapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways mediated by Janus kinase (JAK) and receptor tyrosine kinases, including the receptors of insulin (INS), insulin-like growth factor I (IGF1), nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), platelet-derived growth factor (PDGF) and fibroblast growth factors (FGFs). In growth hormone (GH) signaling, autophosphorylated ('Tyr-813') JAK2 recruits SH2B1, which in turn is phosphorylated by JAK2 on tyrosine residues. These phosphotyrosines form potential binding sites for other signaling proteins. GH also promotes serine/threonine phosphorylation of SH2B1 and these phosphorylated residues may serve to recruit other proteins to the GHR-JAK2-SH2B1 complexes, such as RAC1. In leptin (LEP) signaling, binds to and potentiates the activation of JAK2 by globally enhancing downstream pathways. In response to leptin, binds simultaneously to both, JAK2 and IRS1 or IRS2, thus mediating formation of a complex of JAK2, SH2B1 and IRS1 or IRS2. Mediates tyrosine phosphorylation of IRS1 and IRS2, resulting in activation of the PI 3-kinase pathway. Acts as positive regulator of NGF-mediated activation of the Akt/Forkhead pathway; prolongs NGF-induced phosphorylation of AKT1 on 'Ser-473' and AKT1 enzymatic activity. Enhances the kinase activity of the cytokine receptor-associated tyrosine kinase JAK2 and of other receptor tyrosine kinases, such as FGFR3 and NTRK1. For JAK2, the mechanism seems to involve dimerization of both, SH2B1 and JAK2. Enhances RET phosphorylation and kinase activity. Isoforms seem to be differentially involved in IGF-I and PDGF-induced mitogenesis. -
Tissue specificity
Widely expressed with highest levels in skeletal muscle and ovary. -
Sequence similarities
Belongs to the SH2B adapter family.
Contains 1 PH domain.
Contains 1 SH2 domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues in response to receptor kinase stimulation. Phosphorylated by RET. -
Cellular localization
Cytoplasm. Membrane. Nucleus. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 25970 Human
- Entrez Gene: 20399 Mouse
- Entrez Gene: 89817 Rat
- Omim: 608937 Human
- SwissProt: Q9NRF2 Human
- SwissProt: Q91ZM2 Mouse
- SwissProt: Q62985 Rat
- Unigene: 15744 Human
see all -
Alternative names
- DKFZp547G1110 antibody
- FLJ30542 antibody
- KIAA1299 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SH2B1/PSM antibody (ab196575)Immunofluorescence analysis of U20S cells labeling SH2B1/PSM with ab196575 at a 1/50 dilution. Blue: DAPI for nuclear staining.
Protocols
Datasheets and documents
References (2)
ab196575 has been referenced in 2 publications.
- Wang S et al. SH2B1 promotes epithelial-mesenchymal transition through the IRS1/ß-catenin signaling axis in lung adenocarcinoma. Mol Carcinog 57:640-652 (2018). WB, IHC-P, ICC/IF ; Human . PubMed: 29380446
- Wang S et al. SH2B1 promotes NSCLC cell proliferation through PI3K/Akt/mTOR signaling cascade. Cancer Cell Int 18:132 (2018). PubMed: 30202243
