Product nameAnti-SH2B1/PSM antibody
See all SH2B1/PSM primary antibodies
DescriptionRabbit polyclonal to SH2B1/PSM
ab196575 detects endogenous level of total SH2B1/PSM protein.
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant fragment corresponding to Human SH2B1/PSM (internal sequence).
Database link: Q9NRF2
- Extracts of 22RV-1, SW620, HT-29, MFC7 and cell lines; mouse kidney, brain, intestines and testis tissues; U20S cells.
This product was previously labelled as SH2B1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
PBS is without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 79 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionAdapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways mediated by Janus kinase (JAK) and receptor tyrosine kinases, including the receptors of insulin (INS), insulin-like growth factor I (IGF1), nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), platelet-derived growth factor (PDGF) and fibroblast growth factors (FGFs). In growth hormone (GH) signaling, autophosphorylated ('Tyr-813') JAK2 recruits SH2B1, which in turn is phosphorylated by JAK2 on tyrosine residues. These phosphotyrosines form potential binding sites for other signaling proteins. GH also promotes serine/threonine phosphorylation of SH2B1 and these phosphorylated residues may serve to recruit other proteins to the GHR-JAK2-SH2B1 complexes, such as RAC1. In leptin (LEP) signaling, binds to and potentiates the activation of JAK2 by globally enhancing downstream pathways. In response to leptin, binds simultaneously to both, JAK2 and IRS1 or IRS2, thus mediating formation of a complex of JAK2, SH2B1 and IRS1 or IRS2. Mediates tyrosine phosphorylation of IRS1 and IRS2, resulting in activation of the PI 3-kinase pathway. Acts as positive regulator of NGF-mediated activation of the Akt/Forkhead pathway; prolongs NGF-induced phosphorylation of AKT1 on 'Ser-473' and AKT1 enzymatic activity. Enhances the kinase activity of the cytokine receptor-associated tyrosine kinase JAK2 and of other receptor tyrosine kinases, such as FGFR3 and NTRK1. For JAK2, the mechanism seems to involve dimerization of both, SH2B1 and JAK2. Enhances RET phosphorylation and kinase activity. Isoforms seem to be differentially involved in IGF-I and PDGF-induced mitogenesis.
Tissue specificityWidely expressed with highest levels in skeletal muscle and ovary.
Sequence similaritiesBelongs to the SH2B adapter family.
Contains 1 PH domain.
Contains 1 SH2 domain.
modificationsPhosphorylated on tyrosine residues in response to receptor kinase stimulation. Phosphorylated by RET.
Cellular localizationCytoplasm. Membrane. Nucleus. Shuttles between the nucleus and the cytoplasm.
- Information by UniProt
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This product has been referenced in:
- Wang S et al. SH2B1 promotes epithelial-mesenchymal transition through the IRS1/ß-catenin signaling axis in lung adenocarcinoma. Mol Carcinog 57:640-652 (2018). WB, IHC-P, ICC/IF ; Human . Read more (PubMed: 29380446) »
- Wang S et al. SH2B1 promotes NSCLC cell proliferation through PI3K/Akt/mTOR signaling cascade. Cancer Cell Int 18:132 (2018). Read more (PubMed: 30202243) »