Overview

  • Product name

  • Description

    Rabbit polyclonal to SH2B1/PSM
  • Host species

    Rabbit
  • Specificity

    ab196575 detects endogenous level of total SH2B1/PSM protein.

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to Human SH2B1/PSM (internal sequence).
    Database link: Q9NRF2

  • Positive control

    • Extracts of 22RV-1, SW620, HT-29, MFC7 and cell lines; mouse kidney, brain, intestines and testis tissues; U20S cells.
  • General notes

     This product was previously labelled as SH2B1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab196575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 79 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Adapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways mediated by Janus kinase (JAK) and receptor tyrosine kinases, including the receptors of insulin (INS), insulin-like growth factor I (IGF1), nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), platelet-derived growth factor (PDGF) and fibroblast growth factors (FGFs). In growth hormone (GH) signaling, autophosphorylated ('Tyr-813') JAK2 recruits SH2B1, which in turn is phosphorylated by JAK2 on tyrosine residues. These phosphotyrosines form potential binding sites for other signaling proteins. GH also promotes serine/threonine phosphorylation of SH2B1 and these phosphorylated residues may serve to recruit other proteins to the GHR-JAK2-SH2B1 complexes, such as RAC1. In leptin (LEP) signaling, binds to and potentiates the activation of JAK2 by globally enhancing downstream pathways. In response to leptin, binds simultaneously to both, JAK2 and IRS1 or IRS2, thus mediating formation of a complex of JAK2, SH2B1 and IRS1 or IRS2. Mediates tyrosine phosphorylation of IRS1 and IRS2, resulting in activation of the PI 3-kinase pathway. Acts as positive regulator of NGF-mediated activation of the Akt/Forkhead pathway; prolongs NGF-induced phosphorylation of AKT1 on 'Ser-473' and AKT1 enzymatic activity. Enhances the kinase activity of the cytokine receptor-associated tyrosine kinase JAK2 and of other receptor tyrosine kinases, such as FGFR3 and NTRK1. For JAK2, the mechanism seems to involve dimerization of both, SH2B1 and JAK2. Enhances RET phosphorylation and kinase activity. Isoforms seem to be differentially involved in IGF-I and PDGF-induced mitogenesis.
  • Tissue specificity

    Widely expressed with highest levels in skeletal muscle and ovary.
  • Sequence similarities

    Belongs to the SH2B adapter family.
    Contains 1 PH domain.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues in response to receptor kinase stimulation. Phosphorylated by RET.
  • Cellular localization

    Cytoplasm. Membrane. Nucleus. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp547G1110 antibody
    • FLJ30542 antibody
    • KIAA1299 antibody
    • PH and SH2 domain-containing signaling mediator antibody
    • Pro-rich antibody
    • Pro-rich, PH and SH2 domain-containing signaling mediator antibody
    • PSM antibody
    • SH2 B antibody
    • SH2 domain-containing protein 1B antibody
    • SH2 domain-containing putative adapter SH2-B antibody
    • SH2-B signaling protein antibody
    • SH2B antibody
    • SH2B adapter protein 1 antibody
    • SH2B adaptor protein 1 antibody
    • Sh2b1 antibody
    • SH2B1 protein antibody
    • SH2B1_HUMAN antibody
    see all

Images

  • Immunofluorescence analysis of U20S cells labeling SH2B1/PSM with ab196575 at a 1/50 dilution. Blue: DAPI for nuclear staining.

References

This product has been referenced in:

  • Wang S  et al. SH2B1 promotes epithelial-mesenchymal transition through the IRS1/ß-catenin signaling axis in lung adenocarcinoma. Mol Carcinog 57:640-652 (2018). WB, IHC-P, ICC/IF ; Human . Read more (PubMed: 29380446) »
  • Wang S  et al. SH2B1 promotes NSCLC cell proliferation through PI3K/Akt/mTOR signaling cascade. Cancer Cell Int 18:132 (2018). Read more (PubMed: 30202243) »
See all 2 Publications for this product

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