1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Inhibits estrogen-induced cell proliferation by competing with PLCG for binding to ESR1, blocking the effect of estrogen on PLCG and repressing estrogen-induced proliferation. May play a role in T-cell development and function.
Ubiquitously expressed. Aberrantly expressed in some cancers.
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded Human kidney tissue labeling SH2D4A with ab170797 at a 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot - Anti-SH2D4A antibody - N-terminal (ab170797)
Anti-SH2D4A antibody - N-terminal (ab170797) at 1/100 dilution + NCI-H292 cell line lysates at 35 µg