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Rabbit polyclonal to SH3BGRL
Predicted to work with:
Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig, Zebrafish
A synthetic peptide corresponding to a region within amino acids 66-115 of Human SH3BGRL (LPPQIFNESQ YRGDYDAFFE ARENNAVYAF LGLTAPPGSK EAEVQAKQQA)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 13 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
Belongs to the SH3BGR family.
Information by UniProt
MGC 117402 antibody
OTTHUMP 00000023609 antibody
SH3 binding domain glutamic acid rich protein like antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3BGRL antibody (ab80865)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling SH3BGRL with ab80865 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - SH3BGRL. Right - Merge.
Western blot - Anti-SH3BGRL antibody (ab80865)
Anti-SH3BGRL antibody (ab80865) at 1 µg/ml + MCF7 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG diluted in 1/50000 - 1/100000. Developed using the ECL technique. Predicted band size: 13 kDa
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"