Overview

  • Product name
    Anti-SH3BGRL3 antibody
    See all SH3BGRL3 primary antibodies
  • Description
    Rabbit polyclonal to SH3BGRL3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: SEVTRILDGK RIQYQLVDIS QDNALRDEMR ALAGNPKATP PQIVNGDQYC GDYELFVEAV EQNTLQEFLK LA, corresponding to amino acids 22-93 of Human SH3BGRL3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human smooth muscle tissue; RT4 cell lysates and Human liver and tonsil tissue lysates; U2OS cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixed/permeabilized with PFA/Triton X-100

Target

  • Function
    Could act as a modulator of glutaredoxin biological activity.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the SH3BGR family.
    Contains 1 glutaredoxin domain.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • epididymis secretory protein Li 297 antibody
      • HEL-S-297 antibody
      • SH3 domain binding glutamic acid rich protein like 3 antibody
      • SH3 domain-binding glutamic acid-rich-like protein 3 antibody
      • SH3 domain-binding protein 1 antibody
      • Sh3bgrl3 antibody
      • SH3BGRL3 like protein antibody
      • SH3BP-1 antibody
      • SH3L3_HUMAN antibody
      • TIP B1 antibody
      • TIP-B1 antibody
      • TNF inhibitory protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3BGRL3 antibody (ab122138)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3BGRL3 antibody (ab122138)
      ab122138, at 1/600 dilution, staining SH3BGRL3 in Human smooth muscle tissue by Immunohistochemistry using paraffin-embedded tissue.
    • Western blot - Anti-SH3BGRL3 antibody (ab122138)
      Western blot - Anti-SH3BGRL3 antibody (ab122138)
      All lanes : Anti-SH3BGRL3 antibody (ab122138) at 1/250 dilution

      Lane 1 : RT4 cell lysates
      Lane 2 : U251MG cell lysates
      Lane 3 : Human plasma
      Lane 4 : Human liver tissue lysate
      Lane 5 : Human tonsil tissue lysate
    • Immunocytochemistry/ Immunofluorescence - Anti-SH3BGRL3 antibody (ab122138)
      Immunocytochemistry/ Immunofluorescence - Anti-SH3BGRL3 antibody (ab122138)
      ab122138 at 4 µg/ml staining SH3BGRL3 in PFA/Triton X-100 fixed/permeabilized U2OS cells by Immunofluorescence. Antibody staining is shown in green

    References

    ab122138 has not yet been referenced specifically in any publications.

    Publishing research using ab122138? Please let us know so that we can cite the reference in this datasheet.

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