Anti-SH3BGRL3 antibody (ab122138)
Key features and details
- Rabbit polyclonal to SH3BGRL3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SH3BGRL3 antibody -
Description
Rabbit polyclonal to SH3BGRL3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: SEVTRILDGK RIQYQLVDIS QDNALRDEMR ALAGNPKATP PQIVNGDQYC GDYELFVEAV EQNTLQEFLK LA, corresponding to amino acids 22-93 of Human SH3BGRL3
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Positive control
- Human smooth muscle tissue; RT4 cell lysates and Human liver and tonsil tissue lysates; U2OS cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab122138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.04 - 0.4 µg/ml. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixed/permeabilized with PFA/Triton X-100 |
Target
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Function
Could act as a modulator of glutaredoxin biological activity. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the SH3BGR family.
Contains 1 glutaredoxin domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 83442 Human
- SwissProt: Q9H299 Human
- Unigene: 109051 Human
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Alternative names
- epididymis secretory protein Li 297 antibody
- HEL-S-297 antibody
- SH3 domain binding glutamic acid rich protein like 3 antibody
see all
Images
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Western blot - Anti-SH3BGRL3 antibody (ab122138)All lanes : Anti-SH3BGRL3 antibody (ab122138) at 1/250 dilution
Lane 1 : RT4 cell lysates
Lane 2 : U251MG cell lysates
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3BGRL3 antibody (ab122138)ab122138, at 1/600 dilution, staining SH3BGRL3 in Human smooth muscle tissue by Immunohistochemistry using paraffin-embedded tissue. -
Immunocytochemistry/ Immunofluorescence - Anti-SH3BGRL3 antibody (ab122138)ab122138 at 4 µg/ml staining SH3BGRL3 in PFA/Triton X-100 fixed/permeabilized U2OS cells by Immunofluorescence. Antibody staining is shown in green
Protocols
Datasheets and documents
References (0)
ab122138 has not yet been referenced specifically in any publications.
