Anti-SH3BP1 antibody (ab227548)
Key features and details
- Rabbit polyclonal to SH3BP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SH3BP1 antibody -
Description
Rabbit polyclonal to SH3BP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within SH3BP1 (internal sequence). The exact sequence is proprietary.
Database link: Q9Y3L3 -
Positive control
- WB: A431 whole cell lysate. IHC-P: Mouse duodenum tissue; MDA-MB-157 tumor xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 76 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 76 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. This protein binds preferentially to the ABL1 proto-oncogene, SRC and GRB2. Shows GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins. It inhibits PDGF-induced membrane ruffling mediated by Rac. -
Sequence similarities
Contains 1 BAR domain.
Contains 1 Rho-GAP domain. - Information by UniProt
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Database links
- Entrez Gene: 23616 Human
- Entrez Gene: 20401 Mouse
- SwissProt: Q9Y3L3 Human
- SwissProt: P55194 Mouse
- Unigene: 601143 Human
- Unigene: 4462 Mouse
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Alternative names
- 3BP 1 antibody
- 3BP-1 antibody
- 3BP1_HUMAN antibody
see all
Images
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Anti-SH3BP1 antibody (ab227548) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 76 kDa7.5% SDS-PAGE
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Paraffin-embedded mouse duodenum tissue stained for SH3BP1 with ab227548 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded MDA-MB-157 tumor xenograft tissue stained for SH3BP1 with ab227548 at 1/250 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227548 has not yet been referenced specifically in any publications.