Anti-SH3BP2 (phospho S427) antibody (ab2176)
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Overview
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Product nameAnti-SH3BP2 (phospho S427) antibody
See all SH3BP2 primary antibodies -
DescriptionRabbit polyclonal to SH3BP2 (phospho S427)
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host speciesRabbit
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Species reactivity
Predicted to work with: Human
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Immunogen
Synthetic phospho peptide: SFRSFSpFEKPRQ, corresponding to amino acids 422-433 of Human SH3BP2.
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General notes
Phosphorylation of SH3BP2 occurs on Ser 427 for activation. SH3BP2 mediates interactions of huntingtin and MLK2 (mixed lineage kinase).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesAffinity purified chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
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Primary antibody notesPhosphorylation of SH3BP2 occurs on Ser 427 for activation. SH3BP2 mediates interactions of huntingtin and MLK2 (mixed lineage kinase).
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
| Application | Abreviews | Notes |
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| WB | Use at an assay dependent concentration. Predicted molecular weight: 62 kDa. Reacts in Western blotting with a band at about 60kD, that probably corresponds to SH3BP1 (Not tested for phospho specificity by Western). |
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| ELISA | Use at an assay dependent concentration. Tested in peptide ELISA against 0.1 ug of the immunizing peptide. |
Target
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FunctionBinds differentially to the SH3 domains of certain proteins of signal transduction pathways. Binds to phosphatidylinositols; linking the hemopoietic tyrosine kinase fes to the cytoplasmic membrane in a phosphorylation dependent mechanism.
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Tissue specificityExpressed in a variety of tissues including lung, liver, skeletal muscle, kidney and pancreas.
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Involvement in diseaseDefects in SH3BP2 are the cause of cherubism (CRBM) [MIM:118400]. CRBM is an autosomal dominant inherited syndrome characterized by excessive bone degradation of the upper and lower jaws, which often begins around three years of age. It is followed by development of fibrous tissue masses, which causes a characteristic facial swelling.
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Sequence similaritiesContains 1 PH domain.
Contains 1 SH2 domain. - Information by UniProt
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Database links
- Entrez Gene: 6452 Human
- Omim: 602104 Human
- SwissProt: P78314 Human
- Unigene: 167679 Human
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Alternative names
- 3BP-2 antibody
- 3BP2 antibody
- 3BP2_HUMAN antibody
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Images
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Western blot - Anti-SH3BP2 (phospho S427) antibody (ab2176)Western blot analysis is shown using ab2176 to detect endogenous protein present in unstimulated human whole cell lysates. The band as indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B). Comparison to a molecular weight marker indicates a band of ~60 kDa that probably corresponds to human SH3BP2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.
Protocols
Datasheets and documents
References
ab2176 has not yet been referenced specifically in any publications.
