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Signal Transduction Adapters Cytoplasmic
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Anti-SH3BP2 (phospho S427) antibody (ab2176)

This fast track antibody is not yet fully characterized. It is subject to these terms and conditions.
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Western blot - Anti-SH3BP2 (phospho S427) antibody (ab2176)

    Key features and details

    • Rabbit polyclonal to SH3BP2 (phospho S427)
    • Suitable for: WB, ELISA
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-SH3BP2 (phospho S427) antibody
      See all SH3BP2 primary antibodies
    • Description

      Rabbit polyclonal to SH3BP2 (phospho S427)

      This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic phospho peptide: SFRSFSpFEKPRQ, corresponding to amino acids 422-433 of Human SH3BP2.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes


      Phosphorylation of SH3BP2 occurs on Ser 427 for activation. SH3BP2 mediates interactions of huntingtin and MLK2 (mixed lineage kinase).

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.424% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Affinity purified chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
    • Primary antibody notes

      Phosphorylation of SH3BP2 occurs on Ser 427 for activation. SH3BP2 mediates interactions of huntingtin and MLK2 (mixed lineage kinase).
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Adapters
      • Cytoplasmic
      • Signal Transduction
      • Signaling Pathway
      • Lipid Signaling
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.

    Reacts in Western blotting with a band at about 60kD, that probably corresponds to SH3BP1 (Not tested for phospho specificity by Western).

    ELISA
    Use at an assay dependent concentration.

    Tested in peptide ELISA against 0.1 ug of the immunizing peptide.

    Notes
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.

    Reacts in Western blotting with a band at about 60kD, that probably corresponds to SH3BP1 (Not tested for phospho specificity by Western).

    ELISA
    Use at an assay dependent concentration.

    Tested in peptide ELISA against 0.1 ug of the immunizing peptide.

    Target

    • Function

      Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. Binds to phosphatidylinositols; linking the hemopoietic tyrosine kinase fes to the cytoplasmic membrane in a phosphorylation dependent mechanism.
    • Tissue specificity

      Expressed in a variety of tissues including lung, liver, skeletal muscle, kidney and pancreas.
    • Involvement in disease

      Defects in SH3BP2 are the cause of cherubism (CRBM) [MIM:118400]. CRBM is an autosomal dominant inherited syndrome characterized by excessive bone degradation of the upper and lower jaws, which often begins around three years of age. It is followed by development of fibrous tissue masses, which causes a characteristic facial swelling.
    • Sequence similarities

      Contains 1 PH domain.
      Contains 1 SH2 domain.
    • Target information above from: UniProt accession P78314 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6452 Human
      • Omim: 602104 Human
      • SwissProt: P78314 Human
      • Unigene: 167679 Human
      • Alternative names

        • 3BP-2 antibody
        • 3BP2 antibody
        • 3BP2_HUMAN antibody
        • Abl SH3 binding protein 2 antibody
        • Cherubism antibody
        • CRBM antibody
        • CRPM antibody
        • FLJ42079 antibody
        • FLJ54978 antibody
        • RES4-23 antibody
        • SH3 domain binding protein 2 antibody
        • SH3 domain-binding protein 2 antibody
        • Sh3bp2 antibody
        • TNFAIP3 interacting protein 2 antibody
        see all

      Images

      This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

      • Western blot - Anti-SH3BP2 (phospho S427) antibody (ab2176)
        Western blot - Anti-SH3BP2 (phospho S427) antibody (ab2176)
        Western blot analysis is shown using ab2176 to detect endogenous protein present in unstimulated human whole cell lysates.  The band as indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B).  Comparison to a molecular weight marker indicates a band of ~60 kDa that probably corresponds to human SH3BP2 protein.  The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. 

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab2176? Please let us know so that we can cite the reference in this datasheet.

      ab2176 has not yet been referenced specifically in any publications.

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