Anti-SH3BP4 antibody (ab106609)

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Western blot - Anti-SH3BP4 antibody (ab106609)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3BP4 antibody (ab106609)

Key features and details

  • Rabbit polyclonal to SH3BP4
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-SH3BP4 antibody

  • Description

    Rabbit polyclonal to SH3BP4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to 18 amino acids near the C terminal end of Human SH3BP4 (NP_055336).

  • Positive control

    • Rat lung tissue lysate. Human lung tissue.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at 4°C (stable for up to 12 months).

  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 107 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Functions in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin-mediated endocytosis.

  • Tissue specificity

    Expressed in all tissues tested with higher expression in pancreas. Expressed by retinal pigment epithelial cells (at protein level).

  • Sequence similarities

    Contains 1 SH3 domain.

  • Domain

    The SH3 domain mediates localization to the clathrin-coated pits and vesicles. The SH3 domain mediates interaction with DNM2 and the cytoplasmic part of TFRC with a lower affinity.

  • Post-translational
    modifications

    Phosphorylated upon EGF stimulation. Phosphorylation prevents interaction with DNM2.

  • Cellular localization

    Membrane > clathrin-coated pit. Cytoplasmic vesicle > clathrin-coated vesicle. Nucleus. Specifically associated with transferrin receptor-containing clathrin-coated pits and clathrin-coated vesicles. May also localize to the nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • BOG25 antibody
    • EH binding protein antibody
    • EH binding protein 10 antibody
    • EH-binding protein 10 antibody
    • EHB10 antibody
    • SH3 domain binding protein 4 antibody
    • SH3 domain-binding protein 4 antibody
    • SH3B4_HUMAN antibody
    • sh3bp4 antibody
    • Transferrin receptor trafficking protein antibody
    • Transferrin receptor-trafficking protein antibody
    • TTP antibody
    see all

Images

  • Western blot - Anti-SH3BP4 antibody (ab106609)
    Western blot - Anti-SH3BP4 antibody (ab106609)
    Lane 1 : Anti-SH3BP4 antibody (ab106609) at 1 µg/ml
    Lane 2 : Anti-SH3BP4 antibody (ab106609) at 2 µg/ml

    All lanes : Rat lung tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 107 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3BP4 antibody (ab106609)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3BP4 antibody (ab106609)
    ab106609, at 5µg/ml, staining SH3BP4 in paraffin embedded Human lung tissue by Immunohistochemistry.

References (0)

Publishing research using ab106609? Please let us know so that we can cite the reference in this datasheet.

ab106609 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Submit a question

Question

Regarding ab57005 - will this antibody reactive with mouse or rat? Will it detect both isoforms?
Regarding ab106609 - Will this antibody react with both isoforms?

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Answer

Thank you for contacting Abcam regarding our SH3BP4 antibodies.


Regarding ab106609, this antibody is specific for isoform 1.


The immunogen of antibody ab57005 shares 97% identity with the mouse and rat sequences, thus it is likely the antibody will cross react. Additionally the immunogen is 97% homologous with both isoforms. Since this antibody is not tested for these species, I am happy to offer the following:


I can offer a discount off a future purchase if you buy ab57005 now, test it in mouse and/or rat and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab57005 in mouse and/or rat. I will then send a discount code. This code must be issued before purchasing ab57005 so please wait for my reply before ordering.

2. Purchase ab57005 either by phone, fax, or online (www.abcam.com).

3. Test it in mouse and/or rat.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab57005 turns out to be unsuitable for mouse and/or rat, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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