Key features and details
- Rabbit polyclonal to SH3BP4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SH3BP4 antibody
DescriptionRabbit polyclonal to SH3BP4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to 18 amino acids near the C terminal end of Human SH3BP4 (NP_055336).
- Rat lung tissue lysate. Human lung tissue.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months).
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab106609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 107 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionFunctions in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin-mediated endocytosis.
Tissue specificityExpressed in all tissues tested with higher expression in pancreas. Expressed by retinal pigment epithelial cells (at protein level).
Sequence similaritiesContains 1 SH3 domain.
DomainThe SH3 domain mediates localization to the clathrin-coated pits and vesicles. The SH3 domain mediates interaction with DNM2 and the cytoplasmic part of TFRC with a lower affinity.
modificationsPhosphorylated upon EGF stimulation. Phosphorylation prevents interaction with DNM2.
Cellular localizationMembrane > clathrin-coated pit. Cytoplasmic vesicle > clathrin-coated vesicle. Nucleus. Specifically associated with transferrin receptor-containing clathrin-coated pits and clathrin-coated vesicles. May also localize to the nucleus.
- Information by UniProt
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Lane 1 : Anti-SH3BP4 antibody (ab106609) at 1 µg/ml
Lane 2 : Anti-SH3BP4 antibody (ab106609) at 2 µg/ml
All lanes : Rat lung tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 107 kDa
ab106609, at 5µg/ml, staining SH3BP4 in paraffin embedded Human lung tissue by Immunohistochemistry.
ab106609 has not yet been referenced specifically in any publications.