Anti-SH3BP5 antibody (ab222860)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SH3BP5 antibody
See all SH3BP5 primary antibodies -
Description
Rabbit polyclonal to SH3BP5 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment corresponding to Human SH3BP5 aa 49-455.
Sequence:LEKLNQSTDDINRRETELEDARQKFRSVLVEATVKLDELVKKIGKAVEDS KPYWEARRVARQAQLEAQKATQDFQRATEVLRAAKETISLAEQRLLEDDK RQFDSAWQEMLNHATQRVMEAEQTKTRSELVHKETAARYNAAMGRMRQLE KKLKRAINKSKPYFELKAKYYVQLEQLKKTVDDLQAKLTLAKGEYKMALK NLEMISDEIHERRRSSAMGPRGCGVGAEGSSTSVEDLPGSKPEPDAISVA SEAFEDDSCSNFVSEDDSETQSVSSFSSGPTSPSEMPDQFPAVVRPGSLD LPSPVSLSEFGMMFPVLGPRSECSGASSPECEVERGDRAEGAENKTSDKA NNNRGLSSSSGSGGSSKSQSSTSPEGQALENRMKQLSLQCSKGRDGIIAD IKMVQIG
Database link: O60239 -
Positive control
- WB: Mouse heart, brain and kidney lysates. IHC-P: Human ovarian cancer tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab222860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/200. | |
| WB | 1/2000 - 1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). | |
| IHC-P | 1/20 - 1/200. |
Target
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Function
Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death. -
Tissue specificity
Highly expressed in testis and ovaries. It is also expressed in a variety of tissues including spleen, lymph node, thymus, bone marrow, fetal liver, colon, small intestine and prostate. -
Sequence similarities
Belongs to the SH3BP5 family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 9467 Human
- Entrez Gene: 24056 Mouse
- Entrez Gene: 79566 Mouse
- Entrez Gene: 117186 Rat
- Omim: 605612 Human
- SwissProt: O60239 Human
- SwissProt: Q99LH9 Mouse
- SwissProt: Q9Z131 Mouse
see all -
Alternative names
- 3BP5_HUMAN antibody
- OTTHUMP00000208289 antibody
- OTTHUMP00000208291 antibody
see all
Images
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Western blot - Anti-SH3BP5 antibody (ab222860)All lanes : Anti-SH3BP5 antibody (ab222860) at 1/2000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse kidney lysate
Predicted band size: 51 kDa
Observed band size: 51 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3BP5 antibody (ab222860)Paraffin-embedded human ovarian cancer tissue stained for SH3BP5 using ab222860 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-SH3BP5 antibody (ab222860)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SH3BP5 (green) using ab222860 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Protocols
References
ab222860 has not yet been referenced specifically in any publications.
