Key features and details
- Rabbit polyclonal to SH3BP5
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SH3BP5 antibody
See all SH3BP5 primary antibodies
DescriptionRabbit polyclonal to SH3BP5
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant fragment corresponding to Human SH3BP5 aa 49-455.
LEKLNQSTDDINRRETELEDARQKFRSVLVEATVKLDELVKKIGKAVEDS KPYWEARRVARQAQLEAQKATQDFQRATEVLRAAKETISLAEQRLLEDDK RQFDSAWQEMLNHATQRVMEAEQTKTRSELVHKETAARYNAAMGRMRQLE KKLKRAINKSKPYFELKAKYYVQLEQLKKTVDDLQAKLTLAKGEYKMALK NLEMISDEIHERRRSSAMGPRGCGVGAEGSSTSVEDLPGSKPEPDAISVA SEAFEDDSCSNFVSEDDSETQSVSSFSSGPTSPSEMPDQFPAVVRPGSLD LPSPVSLSEFGMMFPVLGPRSECSGASSPECEVERGDRAEGAENKTSDKA NNNRGLSSSSGSGGSSKSQSSTSPEGQALENRMKQLSLQCSKGRDGIIAD IKMVQIG
Database link: O60239
- WB: Mouse heart, brain and kidney lysates. IHC-P: Human ovarian cancer tissue. ICC/IF: HeLa cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab222860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/2000 - 1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionInhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death.
Tissue specificityHighly expressed in testis and ovaries. It is also expressed in a variety of tissues including spleen, lymph node, thymus, bone marrow, fetal liver, colon, small intestine and prostate.
Sequence similaritiesBelongs to the SH3BP5 family.
- Information by UniProt
- 3BP5_HUMAN antibody
- OTTHUMP00000208289 antibody
- OTTHUMP00000208291 antibody
All lanes : Anti-SH3BP5 antibody (ab222860) at 1/2000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse kidney lysate
Predicted band size: 51 kDa
Observed band size: 51 kDa
Paraffin-embedded human ovarian cancer tissue stained for SH3BP5 using ab222860 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SH3BP5 (green) using ab222860 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
ab222860 has not yet been referenced specifically in any publications.