Product nameAnti-SH3D20 antibody [EPR12250]
See all SH3D20 primary antibodies
DescriptionRabbit monoclonal [EPR12250] to SH3D20
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Recombinant fragment within Human SH3D20 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q6ZUM4
- HepG2, A549 and BxPC-3 cell lysates; Human breast carcinoma tissue; Human pancreas tissue; BxPC-3 cells and permeabilized A549 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 98 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1.
Tissue specificityExpressed in germinal center B-cell, spleen, chronic lymphocytic leukemia, pancreatic cancer and lung cancer.
Sequence similaritiesContains 1 PH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain.
Contains 3 WW domains.
Cellular localizationCytoplasm. Membrane.
- Information by UniProt
- ARHGAP27 antibody
- CAMGAP1 antibody
- CIN85-associated multi-domain-containing Rho GTPase-activating protein 1 antibody
All lanes : Anti-SH3D20 antibody [EPR12250] (ab171973) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : BxPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 98 kDa
Note: primary antibody diluted in BSA.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling SH3D20 with ab171973 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling SH3D20 with ab171973 at 1/100 dilution.
Immunofluorescent analysis of BxPC-3 cells labeling SH3D20 with ab171973 (green) at 1/50 dilution. DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized A549 cells labeling SH3D20 with ab171973 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).
ab171973 has not yet been referenced specifically in any publications.