Overview

  • Product name
    Anti-SH3D20 antibody [EPR12250]
    See all SH3D20 primary antibodies
  • Description
    Rabbit Monoclonal [EPR12250] to SH3D20
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human SH3D20 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6ZUM4

  • Positive control
    • HepG2, A549 and BxPC-3 cell lysates; Human breast carcinoma tissue; Human pancreas tissue; BxPC-3 cells and permeabilized A549 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 98 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1.
    • Tissue specificity
      Expressed in germinal center B-cell, spleen, chronic lymphocytic leukemia, pancreatic cancer and lung cancer.
    • Sequence similarities
      Contains 1 PH domain.
      Contains 1 Rho-GAP domain.
      Contains 1 SH3 domain.
      Contains 3 WW domains.
    • Cellular localization
      Cytoplasm. Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARHGAP27 antibody
      • CAMGAP1 antibody
      • CIN85-associated multi-domain-containing Rho GTPase-activating protein 1 antibody
      • PP905 antibody
      • RHG27_HUMAN antibody
      • Rho GTPase-activating protein 27 antibody
      • Rho-type GTPase-activating protein 27 antibody
      • SH3 domain containing 20 antibody
      • SH3 domain containing protein 20 antibody
      • SH3D20 antibody
      • SH3P20 antibody
      see all

    Images

    • All lanes : Anti-SH3D20 antibody [EPR12250] (ab171973) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : BxPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 98 kDa



      Note: primary antibody diluted in BSA.

    • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling SH3D20 with ab171973 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling SH3D20 with ab171973 at 1/100 dilution.

    • Immunofluorescent analysis of BxPC-3 cells labeling SH3D20 with ab171973 (green) at 1/50 dilution. DAPI nuclear staining (blue).

    • Flow cytometric analysis of permeabilized A549 cells labeling SH3D20 with ab171973 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).

    References

    ab171973 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab171973.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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