Key features and details
- Rabbit polyclonal to SH3GL2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SH3GL2 antibody
See all SH3GL2 primary antibodies
DescriptionRabbit polyclonal to SH3GL2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Human SH3GL2 aa 1-270.
MSVAGLKKQFHKATQKVSEKVGGAEGTKLDDDFKEMERKVDVTSRAVMEI MTKTIEYLQPNPASRAKLSMINTMSKIRGQEKGPGYPQAEALLAEAMLKF GRELGDDCNFGPALGEVGEAMRELSEVKDSLDIEVKQNFIDPLQNLHDKD LREIQHHLKKLEGRRLDFDYKKKRQGKIPDEELRQALEKFDESKEIAESS MFNLLEMDIEQVSQLSALVQAQLEYHKQAVQILQQVTVRLEERIRQASSQ PRREYQPKPRMSLEFPTGDS
Database link: Q99962
- WB: HepG2 whole cell lysate. IHC-P: Human brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab235337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 40 kDa.|
|IHC-P||1/20 - 1/200.|
FunctionImplicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature.
Tissue specificityBrain, mostly in frontal cortex. Expressed at high level in fetal cerebellum.
Sequence similaritiesBelongs to the endophilin family.
Contains 1 BAR domain.
Contains 1 SH3 domain.
DomainAn N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes. The BAR domain dimer forms a rigid crescent shaped bundle of helices with the pair of second amphipathic helices protruding towards the membrane-binding surface.
Cellular localizationCytoplasm. Membrane. Concentrated in presynaptic nerve terminals in neurons.
- Information by UniProt
- bA335L15.1 (SH3 domain GRB2 like 2) antibody
- CNSA2 antibody
- EEN B1 antibody
Anti-SH3GL2 antibody (ab235337) at 1/1000 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 40 kDa
Paraffin-embedded human brain tissue stained for SH3GL2 with ab235337 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235337 has not yet been referenced specifically in any publications.