Immunocytochemistry/ Immunofluorescence abreview for Anti-SH3GL2 [EPR10214] antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Mouse Cell (Primary hippocampal neurons)
Specification
Primary hippocampal neurons
Permeabilization
Yes - 0.05%Tx100 + 0.05% Tween20 in PBS
Fixative
Paraformaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% BSA/ PBS
Dilution
1/150

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 594 Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1500

Additional data

Additional Notes
Fixatin was done for 15min. with 2% PFA at room temperature

Ms. Eva Borger

Verified customer

Submitted Apr 16 2014

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