Key features and details
- Rabbit polyclonal to SH3GLB2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SH3GLB2 antibody
See all SH3GLB2 primary antibodies
DescriptionRabbit polyclonal to SH3GLB2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human SH3GLB2 aa 1-395.
Database link: Q9NR46
- WB: HeLa, U-87 MG, THP-1, A549, MCF7 and A431 whole cell lysates; Mouse gonadal, lung and liver lysates. IHC-P: Human placenta tissue.
This product was previously labelled as SPS2L
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
- HeLa whole cell lysate (ab150035)
- Mouse lung normal tissue lysate - total protein (ab29297)
- Mouse liver tissue lysate - total protein (ab29301)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- A431 whole cell lysate (ab30132)
- MCF7 whole cell lysate (ab3871)
- A431 whole cell lysate (ab7909)
- THP1 whole cell lysate (ab7913)
Our Abpromise guarantee covers the use of ab235352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 44 kDa.|
|IHC-P||1/20 - 1/200.|
Tissue specificityDetected in skeletal muscle, adipocyte, brain, lung, colon and mammary gland.
Sequence similaritiesBelongs to the endophilin family.
Contains 1 BAR domain.
Contains 1 SH3 domain.
- Information by UniProt
- Endophilin B2 antibody
- Endophilin-B2 antibody
- OTTHUMP00000022320 antibody
All lanes : Anti-SH3GLB2 antibody (ab235352) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Mouse gonadal tissue lysate
Lane 3 : Mouse lung tissue lysate
Lane 4 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 5 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 6 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 7 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 8 : Mouse liver tissue lysate
Lane 9 : A431 (human epidermoid carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 44 kDa
Paraffin-embedded human placenta tissue stained for SPS2L/SPATS2L using ab235352 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235352 has not yet been referenced specifically in any publications.