Overview

  • Product name
    Anti-SH3KBP1 antibody [EPR7880(2)]
    See all SH3KBP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR7880(2)] to SH3KBP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human SH3KBP1 (Uniprot ID Q96B97).

  • Positive control
    • Human spleen, fetal thymus, fetal brain and Jurkat lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR7880(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through a association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit (By similarity). May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis.
    • Tissue specificity
      Ubiquitously expressed. Also expressed in some cancer cell lines.
    • Sequence similarities
      Contains 3 SH3 domains.
    • Domain
      The SH3 domains mediate interaction with SHKBP1.
    • Post-translational
      modifications
      Monoubiquitinated by CBL and CBLB after EGF stimulation; probably on its C-terminus.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cytoplasmic vesicle membrane. Cell junction > synapse > synaptosome. Cell junction > focal adhesion. Localized in endocytic vesicles containing clustered receptors. Colocalizes with ASAP1 in vesicular structures. Colocalized with actin microfilaments and focal adhesions (By similarity). Colocalized with MAGI2 in synaptosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • c Cbl interacting protein antibody
      • Cbl interacting protein antibody
      • Cbl interacting protein of 85 kDa antibody
      • Cbl-interacting protein of 85 kDa antibody
      • CD2 binding protein 3 antibody
      • CD2-binding protein 3 antibody
      • CD2BP3 antibody
      • CIN 85 antibody
      • CIN85 antibody
      • GIG 10 antibody
      • GIG10 antibody
      • HSB 1 antibody
      • HSB-1 antibody
      • HSB1 antibody
      • Human Src family kinase binding protein 1 antibody
      • Human Src family kinase-binding protein 1 antibody
      • MIG 18 antibody
      • MIG18 antibody
      • Migration inducing gene 18 antibody
      • Migration inducing gene 18 protein antibody
      • SH3 domain kinase binding protein 1 antibody
      • SH3 domain-containing kinase-binding protein 1 antibody
      • SH3BP 1 antibody
      • SH3K1_HUMAN antibody
      • SH3KBP1 antibody
      • Src family kinase binding protein 1 antibody
      • src related kinase binding protein 1 antibody
      see all

    Images

    • All lanes : Anti-SH3KBP1 antibody [EPR7880(2)] (ab154858) at 1/1000 dilution

      Lane 1 : Human spleen tissue lysate
      Lane 2 : Human fetal thymus tissue lysate
      Lane 3 : Human fetal brain tissue lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 73 kDa

    References

    ab154858 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab154858.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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