Product nameAnti-SH3KBP1 antibody [EPR7880(2)]
See all SH3KBP1 primary antibodies
DescriptionRabbit monoclonal [EPR7880(2)] to SH3KBP1
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide, corresponding to residues in Human SH3KBP1 (Uniprot ID Q96B97).
- Human spleen, fetal thymus, fetal brain and Jurkat lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab154858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 73 kDa.|
FunctionAdapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through a association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit (By similarity). May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis.
Tissue specificityUbiquitously expressed. Also expressed in some cancer cell lines.
Sequence similaritiesContains 3 SH3 domains.
DomainThe SH3 domains mediate interaction with SHKBP1.
modificationsMonoubiquitinated by CBL and CBLB after EGF stimulation; probably on its C-terminus.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasmic vesicle membrane. Cell junction > synapse > synaptosome. Cell junction > focal adhesion. Localized in endocytic vesicles containing clustered receptors. Colocalizes with ASAP1 in vesicular structures. Colocalized with actin microfilaments and focal adhesions (By similarity). Colocalized with MAGI2 in synaptosomes.
- Information by UniProt
- c Cbl interacting protein antibody
- Cbl interacting protein antibody
- Cbl interacting protein of 85 kDa antibody
All lanes : Anti-SH3KBP1 antibody [EPR7880(2)] (ab154858) at 1/1000 dilution
Lane 1 : Human spleen tissue lysate
Lane 2 : Human fetal thymus tissue lysate
Lane 3 : Human fetal brain tissue lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 73 kDa
ab154858 has not yet been referenced specifically in any publications.