Overview

  • Product name

    Anti-SH3KBP1 antibody [EPR7881(2)]
    See all SH3KBP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7881(2)] to SH3KBP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SH3KBP1 aa 400-500. The exact sequence is proprietary.

  • Positive control

    • Jurkat and THP1 cell lysates; Human colonic adenocarcinoma and tonsil tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7881(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through a association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit (By similarity). May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis.
    • Tissue specificity

      Ubiquitously expressed. Also expressed in some cancer cell lines.
    • Sequence similarities

      Contains 3 SH3 domains.
    • Domain

      The SH3 domains mediate interaction with SHKBP1.
    • Post-translational
      modifications

      Monoubiquitinated by CBL and CBLB after EGF stimulation; probably on its C-terminus.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cytoplasmic vesicle membrane. Cell junction > synapse > synaptosome. Cell junction > focal adhesion. Localized in endocytic vesicles containing clustered receptors. Colocalizes with ASAP1 in vesicular structures. Colocalized with actin microfilaments and focal adhesions (By similarity). Colocalized with MAGI2 in synaptosomes.
    • Information by UniProt
    • Database links

    • Alternative names

      • c Cbl interacting protein antibody
      • Cbl interacting protein antibody
      • Cbl interacting protein of 85 kDa antibody
      • Cbl-interacting protein of 85 kDa antibody
      • CD2 binding protein 3 antibody
      • CD2-binding protein 3 antibody
      • CD2BP3 antibody
      • CIN 85 antibody
      • CIN85 antibody
      • GIG 10 antibody
      • GIG10 antibody
      • HSB 1 antibody
      • HSB-1 antibody
      • HSB1 antibody
      • Human Src family kinase binding protein 1 antibody
      • Human Src family kinase-binding protein 1 antibody
      • MIG 18 antibody
      • MIG18 antibody
      • Migration inducing gene 18 antibody
      • Migration inducing gene 18 protein antibody
      • SH3 domain kinase binding protein 1 antibody
      • SH3 domain-containing kinase-binding protein 1 antibody
      • SH3BP 1 antibody
      • SH3K1_HUMAN antibody
      • SH3KBP1 antibody
      • Src family kinase binding protein 1 antibody
      • src related kinase binding protein 1 antibody
      see all

    Images

    • All lanes : Anti-SH3KBP1 antibody [EPR7881(2)] (ab151574) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : THP1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 73 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling SH3KBP1 with ab151574 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling SH3KBP1 with ab151574 at 1/50 dilution.

    References

    This product has been referenced in:

    • Hartmann H  et al. Proteomics and C9orf72 neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity. Life Sci Alliance 1:e201800070 (2018). Read more (PubMed: 30456350) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab151574.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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