Overview

  • Product name

    Anti-SH3KBP1 antibody - N-terminal
    See all SH3KBP1 primary antibodies
  • Description

    Goat polyclonal to SH3KBP1 - N-terminal
  • Host species

    Goat
  • Specificity

    ab223208 is expected to recognize isoform a (NP_114098.1) only.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human SH3KBP1 aa 2-14 (N terminal) (Cysteine residue). (NP_114098.1).
    Sequence:

    VEAIVEFDYQAQH-C


    Database link: Q96B97

  • Positive control

    • IHC-P: Human spleen tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab223208 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through a association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit (By similarity). May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis.
  • Tissue specificity

    Ubiquitously expressed. Also expressed in some cancer cell lines.
  • Sequence similarities

    Contains 3 SH3 domains.
  • Domain

    The SH3 domains mediate interaction with SHKBP1.
  • Post-translational
    modifications

    Monoubiquitinated by CBL and CBLB after EGF stimulation; probably on its C-terminus.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cytoplasmic vesicle membrane. Cell junction > synapse > synaptosome. Cell junction > focal adhesion. Localized in endocytic vesicles containing clustered receptors. Colocalizes with ASAP1 in vesicular structures. Colocalized with actin microfilaments and focal adhesions (By similarity). Colocalized with MAGI2 in synaptosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • c Cbl interacting protein antibody
    • Cbl interacting protein antibody
    • Cbl interacting protein of 85 kDa antibody
    • Cbl-interacting protein of 85 kDa antibody
    • CD2 binding protein 3 antibody
    • CD2-binding protein 3 antibody
    • CD2BP3 antibody
    • CIN 85 antibody
    • CIN85 antibody
    • GIG 10 antibody
    • GIG10 antibody
    • HSB 1 antibody
    • HSB-1 antibody
    • HSB1 antibody
    • Human Src family kinase binding protein 1 antibody
    • Human Src family kinase-binding protein 1 antibody
    • MIG 18 antibody
    • MIG18 antibody
    • Migration inducing gene 18 antibody
    • Migration inducing gene 18 protein antibody
    • SH3 domain kinase binding protein 1 antibody
    • SH3 domain-containing kinase-binding protein 1 antibody
    • SH3BP 1 antibody
    • SH3K1_HUMAN antibody
    • SH3KBP1 antibody
    • Src family kinase binding protein 1 antibody
    • src related kinase binding protein 1 antibody
    see all

Images

  • Paraffin-embedded human spleen tissue stained for SH3KBP1 with ab223208 at 2.5 µg/ml in immunohistochemical analysis, followed by AP-staining.

References

ab223208 has not yet been referenced specifically in any publications.

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