Key features and details
- Rabbit polyclonal to SH3PX1/SNX9
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SH3PX1/SNX9 antibody
See all SH3PX1/SNX9 primary antibodies
DescriptionRabbit polyclonal to SH3PX1/SNX9
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
- WB: HeLa and K562 whole cell lysates. IHC-P: Human thyroid tissue. ICC/IF: HeLa cells.
This product was previously labelled as SH3PX1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab222911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 67 kDa.|
FunctionMay be involved in several stages of intracellular trafficking. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate.
Sequence similaritiesBelongs to the sorting nexin family.
Contains 1 BAR domain.
Contains 1 PX (phox homology) domain.
Contains 1 SH3 domain.
DomainThe PX domain mediates interaction with membranes enriched in phosphatidylinositol phosphate. Has high affinity for phosphatidylinositol-4,5-bisphosphate, but can also bind to membranes enriched in other phosphatidylinositol phosphates.
modificationsUbiquitinated by ITCH.
Phosphorylated on tyrosine residues by TNK2. Phosphorylation promotes its activity in the degradation of EGFR.
Cellular localizationCytoplasmic vesicle membrane. Cell membrane. Cytoplasmic vesicle > clathrin-coated vesicle. Golgi apparatus > trans-Golgi network. Cell projection > ruffle. Cytoplasm. Localized at sites of endocytosis at the cell membrane. Detected on newly formed macropinosomes. Transiently recruited to clathrin-coated pits at a late stage of clathrin-coated vesicle formation. Colocalizes with the actin cytoskeleton at the cell membrane.
- Information by UniProt
- MST 155 antibody
- MST155 antibody
- MSTP 155 antibody
All lanes : Anti-SH3PX1/SNX9 antibody (ab222911) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDa
Paraffin-embedded human thyroid tissue stained for SH3PX1/SNX9 using ab222911 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SH3PX1/SNX9 (green) using ab222911 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
ab222911 has not yet been referenced specifically in any publications.