Anti-SHANK1 antibody (ab154224)
Overview
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Product name
Anti-SHANK1 antibody
See all SHANK1 primary antibodies -
Description
Rabbit polyclonal to SHANK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to a region within amino acids 2100 and 2161 of Human SHANK1 (Q9Y566)
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Positive control
- 293T whole cell lysate, SW480 xenograft tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 225 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction. -
Tissue specificity
Expressed in brain particularly in the amygdala, hippocampus, substantia nigra and thalamus. Isoform 2 seems to be expressed ubiquitously. -
Sequence similarities
Belongs to the SHANK family.
Contains 6 ANK repeats.
Contains 1 PDZ (DHR) domain.
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH3 domain. -
Cellular localization
Cytoplasm. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Postsynaptic density of neuronal cells. - Information by UniProt
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Database links
- Entrez Gene: 50944 Human
- Entrez Gene: 243961 Mouse
- Entrez Gene: 78957 Rat
- Omim: 604999 Human
- SwissProt: Q9Y566 Human
- SwissProt: D3YZU1 Mouse
- SwissProt: Q9WV48 Rat
- Unigene: 274255 Human
see all -
Alternative names
- GKAP/SAPAP interacting protein antibody
- OTTHUMP00000174437 antibody
- SH3 and multiple ankyrin repeat domains 1 antibody
see all
Images
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Western blot - Anti-SHANK1 antibody (ab154224)Anti-SHANK1 antibody (ab154224) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 225 kDa
5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 antibody (ab154224)Immunohistochemical analysis of paraffin embedded Human J5 xenograft tissue labelling SW480 with ab154224 at a 1/1000 dilution.
Protocols
References
ab154224 has not yet been referenced specifically in any publications.
