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    shank1-antibody-ab154224.pdf

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Anti-SHANK1 antibody (ab154224)

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  • SDS
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Western blot - Anti-SHANK1 antibody (ab154224)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 antibody (ab154224)

Key features and details

  • Rabbit polyclonal to SHANK1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SHANK1 antibody
    See all SHANK1 primary antibodies
  • Description

    Rabbit polyclonal to SHANK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 2100 and 2161 of Human SHANK1 (Q9Y566)

  • Positive control

    • 293T whole cell lysate, SW480 xenograft tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Adapters

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 225 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 225 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction.
  • Tissue specificity

    Expressed in brain particularly in the amygdala, hippocampus, substantia nigra and thalamus. Isoform 2 seems to be expressed ubiquitously.
  • Sequence similarities

    Belongs to the SHANK family.
    Contains 6 ANK repeats.
    Contains 1 PDZ (DHR) domain.
    Contains 1 SAM (sterile alpha motif) domain.
    Contains 1 SH3 domain.
  • Cellular localization

    Cytoplasm. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Postsynaptic density of neuronal cells.
  • Target information above from: UniProt accession Q9Y566 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 50944 Human
    • Entrez Gene: 243961 Mouse
    • Entrez Gene: 78957 Rat
    • Omim: 604999 Human
    • SwissProt: Q9Y566 Human
    • SwissProt: D3YZU1 Mouse
    • SwissProt: Q9WV48 Rat
    • Unigene: 274255 Human
    • Unigene: 360368 Mouse
    • Unigene: 225968 Rat
    see all
  • Alternative names

    • GKAP/SAPAP interacting protein antibody
    • OTTHUMP00000174437 antibody
    • SH3 and multiple ankyrin repeat domains 1 antibody
    • SH3 and multiple ankyrin repeat domains protein 1 antibody
    • SH3/ankyrin domain gene 1 antibody
    • SHAN1_HUMAN antibody
    • SHANK 1 antibody
    • Shank1 antibody
    • Shank1a antibody
    • Somatostatin receptor interacting protein antibody
    • Somatostatin receptor-interacting protein antibody
    • SPANK 1 antibody
    • SPANK1 antibody
    • SSTR interacting protein antibody
    • SSTR-interacting protein antibody
    • SSTRIP antibody
    • Synamon antibody
    see all

Images

  • Western blot - Anti-SHANK1 antibody (ab154224)
    Western blot - Anti-SHANK1 antibody (ab154224)
    Anti-SHANK1 antibody (ab154224) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 225 kDa



    5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 antibody (ab154224)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 antibody (ab154224)
    Immunohistochemical analysis of paraffin embedded Human J5 xenograft tissue labelling SW480 with ab154224 at a 1/1000 dilution.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab154224? Please let us know so that we can cite the reference in this datasheet.

ab154224 has not yet been referenced specifically in any publications.

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