Key features and details
- Rabbit polyclonal to SHANK2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SHANK2 antibody
See all SHANK2 primary antibodies
DescriptionRabbit polyclonal to SHANK2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Human intestine tissue and mouse RAW 264.7, Jurkat, HepG2 and COLO 205 cell lysates
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol
PBS without Mg2+, Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 20 µg/ml.|
|WB||1/500 - 1/1000. Predicted molecular weight: 201 kDa.|
FunctionSeems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
Tissue specificityExpressed in epithelial cells (at protein level). All isoforms except isoform 7 are expressed predominantly in brain, with highest levels in olfactory bulb, cerebral cortex, cerebellum, central gray matter and hippocampus. Moderate levels of expression are seen in the caudate putamen, thalamic nuclei and brain stem. In cerebellum primarily expressed in Purkinje cells. Isoform 7 is not expressed in brain but expressed in liver, cholangiocytes and thymus. Isoform 7 is present in pancreas, colonic mucosa and thymocytes (at protein level).
Sequence similaritiesBelongs to the SHANK family.
Contains 1 PDZ (DHR) domain.
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH3 domain.
Developmental stageExpressed during early postnatal brain development, especially in the caudate putamen and thalamic nuclei. Expression in the cerebral cortex, the hippocampus and the cerebellum is moderate to high at P5 and shows a stable expression throughout development. Isoforms 1, 2, 4 and 6 are predominantly expressed in cerebellum up to the age of approximately 3 weeks. Isoform 1 expression decreases during development of cortex but slightly increases in cerebellum.
DomainThe PDZ domain is required for interaction with GRID2, PLCB3, SLC9A3 and CFTR.
Cellular localizationApical cell membrane. Cytoplasm. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, growth cone. Cell projection, dendritic spine. Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Present in the dendritic spines of cerebellar Purkinje cells (By similarity). Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Colocalized with PDE4D to the apical membrane of colonic crypt cells.
- Information by UniProt
- AUTS17 antibody
- Cortactin binding protein 1 antibody
- Cortactin SH3 domain-binding protein antibody
All lanes : Anti-SHANK2 antibody (ab189398) at 1/500 dilution
Lane 1 : mouse RAW 264.7 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : COLO 205 cell lysate
Lane 5 : mouse RAW 264.7 cell lysate with the synthesized peptide
Predicted band size: 201 kDa
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human intestine tissue labeling SHANK2 using ab189398 at 20 µg/ml
ab189398 has not yet been referenced specifically in any publications.