Overview

  • Product name

    Anti-SHANK2 antibody [S23b-6]
    See all SHANK2 primary antibodies
  • Description

    Mouse monoclonal [S23b-6] to SHANK2
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Fusion protein corresponding to Rat SHANK2 aa 84-309. (SH3/PDZ domains) NP_958738.1
    Database link: Q9QX74

  • Positive control

    • ICC/IF: SK-N-BE cells. WB: Rat brain membrane lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from TCS.
  • Clonality

    Monoclonal
  • Clone number

    S23b-6
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab242005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000. Predicted molecular weight: 159 kDa.

Target

  • Function

    Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
  • Tissue specificity

    Expressed in epithelial cells (at protein level). All isoforms except isoform 7 are expressed predominantly in brain, with highest levels in olfactory bulb, cerebral cortex, cerebellum, central gray matter and hippocampus. Moderate levels of expression are seen in the caudate putamen, thalamic nuclei and brain stem. In cerebellum primarily expressed in Purkinje cells. Isoform 7 is not expressed in brain but expressed in liver, cholangiocytes and thymus. Isoform 7 is present in pancreas, colonic mucosa and thymocytes (at protein level).
  • Sequence similarities

    Belongs to the SHANK family.
    Contains 1 PDZ (DHR) domain.
    Contains 1 SAM (sterile alpha motif) domain.
    Contains 1 SH3 domain.
  • Developmental stage

    Expressed during early postnatal brain development, especially in the caudate putamen and thalamic nuclei. Expression in the cerebral cortex, the hippocampus and the cerebellum is moderate to high at P5 and shows a stable expression throughout development. Isoforms 1, 2, 4 and 6 are predominantly expressed in cerebellum up to the age of approximately 3 weeks. Isoform 1 expression decreases during development of cortex but slightly increases in cerebellum.
  • Domain

    The PDZ domain is required for interaction with GRID2, PLCB3, SLC9A3 and CFTR.
  • Cellular localization

    Apical cell membrane. Cytoplasm. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, growth cone. Cell projection, dendritic spine. Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Present in the dendritic spines of cerebellar Purkinje cells (By similarity). Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Colocalized with PDE4D to the apical membrane of colonic crypt cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • AUTS17 antibody
    • Cortactin binding protein 1 antibody
    • Cortactin SH3 domain-binding protein antibody
    • Cortactin-binding protein 1 antibody
    • CortBP1 antibody
    • CTTNBP1 antibody
    • GKAP/SAPAP interacting protein antibody
    • GKAP/SAPAP-interacting protein antibody
    • KIAA1022 antibody
    • Proline rich synapse associated protein 1 antibody
    • Proline-rich synapse-associated protein 1 antibody
    • ProSAP1 antibody
    • SH3 and multiple ankyrin repeat domains 2 antibody
    • SH3 and multiple ankyrin repeat domains protein 2 antibody
    • SHAN2_RAT antibody
    • SHANK antibody
    • Shank2 antibody
    • SPANK-3 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of SK-N-BE (Human neuroblastoma cell line) cells labeling SHANK2 using ab242005 at 1:100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.

    Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 minutes at RT.

    Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min RT, 5 min RT.

    (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab242005 (D) Composite.

  • Anti-SHANK2 antibody [S23b-6] (ab242005) at 1/1000 dilution (2 hours at RT) + Rat brain membrane lysate at 15 µg

    Secondary
    Sheep anti-Mouse IgG-HRP,1 hour at RT

    Predicted band size: 159 kDa



    Block: 1.5% BSA for 30 minutes at RT.

References

ab242005 has not yet been referenced specifically in any publications.

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