Overview

  • Product name

    Anti-SHANK3 antibody [N69/46]
    See all SHANK3 primary antibodies
  • Description

    Mouse monoclonal [N69/46] to SHANK3
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Rat SHANK3 aa 800-900.

  • Positive control

    • ICC/IF: U20S cells. Mouse cerebellum, rat cortex, rat brain membrane and human cortex lysates gave a positive result in WB.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab193307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 186 kDa (predicted molecular weight: 186 kDa).

Target

  • Function

    Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
  • Tissue specificity

    Expressed in the cerebral cortex and the cerebellum.
  • Involvement in disease

    Note=A chromosomal aberration involving SHANK3 is found in patients with chromosome 22q13.3 deletion syndrome. Translocation t(12;22)(q24.1;q13.3) with APPL2/DIP13B.
    Note=Defects in SHANK3 are associated with autism spectrum disorders (ASD). ASD are characterized by impairments in reciprocal social interaction and communication as well as restricted and stereotyped patterns of interest and activities. ASD include forms with moderate to severe cognitive impairment and milder forms with higher cognitive ability (Asperger syndrome).
    Defects in SHANK3 are the cause of schizophrenia type 15 (SCZD15) [MIM:613950]. SCZD15 is a complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder.
  • Sequence similarities

    Contains 6 ANK repeats.
    Contains 1 PDZ (DHR) domain.
    Contains 1 SAM (sterile alpha motif) domain.
    Contains 1 SH3 domain.
  • Cellular localization

    Cytoplasm. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Postsynaptic density of neuronal cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI841104 antibody
    • DEL22q13.3 antibody
    • KIAA1650 antibody
    • Proline rich synapse associated protein 2 antibody
    • Proline-rich synapse-associated protein 2 antibody
    • ProSAP2 antibody
    • PSAP2 antibody
    • SH3 and multiple ankyrin repeat domains 3 antibody
    • SH3 and multiple ankyrin repeat domains protein 3 antibody
    • SH3/ankyrin domain gene 3 antibody
    • SHAN3_HUMAN antibody
    • Shank postsynaptic density protein antibody
    • Shank3 antibody
    • Shank3b antibody
    • SPANK 2 antibody
    • SPANK2 antibody
    see all

Images

  • All lanes : Anti-SHANK3 antibody [N69/46] (ab193307) at 5 µg/ml

    Lane 1 : Cerebellum Mouse Tissue Lysate
    Lane 2 : Rat Cortex
    Lane 3 : Rat brain normal tissue lysate - membrane extract (ab29473)
    Lane 4 : Human Cortex
    Lane 5 : Human Striatum Neuronal cell lysate
    Lane 6 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 186 kDa
    Observed band size: 186 kDa


    Exposure time: 4 minutes
  • ab193307 stained U20S cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab193307 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

References

This product has been referenced in:

See 1 Publication for this product

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