Overview

  • Product name

    Anti-SHANK3 antibody [N69/46] (FITC)
    See all SHANK3 primary antibodies
  • Description

    Mouse monoclonal [N69/46] to SHANK3 (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    No cross-reactivity against SHANK1 or SHANK2.
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Rat SHANK3 aa 840-857.
    Sequence:

    PEKLPGSLRKGIPRTKSV


    Database link: Q9JLU4

  • Positive control

    • COS cell lysate transiently transfected with SHANK3.
  • General notes

    The clone number has been updated from S69-46 to N69/46, both clone numbers name the same antibody clone.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab183420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 190 kDa (predicted molecular weight: 193 kDa).
IHC-P Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
ICC/IF Use a concentration of 1 - 10 µg/ml.
IP Use at an assay dependent concentration.

Target

  • Function

    Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
  • Tissue specificity

    Expressed in the cerebral cortex and the cerebellum.
  • Involvement in disease

    Note=A chromosomal aberration involving SHANK3 is found in patients with chromosome 22q13.3 deletion syndrome. Translocation t(12;22)(q24.1;q13.3) with APPL2/DIP13B.
    Note=Defects in SHANK3 are associated with autism spectrum disorders (ASD). ASD are characterized by impairments in reciprocal social interaction and communication as well as restricted and stereotyped patterns of interest and activities. ASD include forms with moderate to severe cognitive impairment and milder forms with higher cognitive ability (Asperger syndrome).
    Defects in SHANK3 are the cause of schizophrenia type 15 (SCZD15) [MIM:613950]. SCZD15 is a complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder.
  • Sequence similarities

    Contains 6 ANK repeats.
    Contains 1 PDZ (DHR) domain.
    Contains 1 SAM (sterile alpha motif) domain.
    Contains 1 SH3 domain.
  • Cellular localization

    Cytoplasm. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Postsynaptic density of neuronal cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI841104 antibody
    • DEL22q13.3 antibody
    • KIAA1650 antibody
    • Proline rich synapse associated protein 2 antibody
    • Proline-rich synapse-associated protein 2 antibody
    • ProSAP2 antibody
    • PSAP2 antibody
    • SH3 and multiple ankyrin repeat domains 3 antibody
    • SH3 and multiple ankyrin repeat domains protein 3 antibody
    • SH3/ankyrin domain gene 3 antibody
    • SHAN3_HUMAN antibody
    • Shank postsynaptic density protein antibody
    • Shank3 antibody
    • Shank3b antibody
    • SPANK 2 antibody
    • SPANK2 antibody
    see all

References

ab183420 has not yet been referenced specifically in any publications.

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