Key features and details
- Mouse monoclonal [N69/46] to SHANK3 (FITC)
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Product nameAnti-SHANK3 antibody [N69/46] (FITC)
See all SHANK3 primary antibodies
DescriptionMouse monoclonal [N69/46] to SHANK3 (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
SpecificityNo cross-reactivity against SHANK1 or SHANK2.
Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
- COS cell lysate transiently transfected with SHANK3.
The clone number has been updated from S69-46 to N69/46, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab183420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 190 kDa (predicted molecular weight: 193 kDa).|
|IHC-P||Use a concentration of 0.1 - 1 µg/ml.|
|IHC-Fr||Use a concentration of 0.1 - 1 µg/ml.|
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|
|IP||Use at an assay dependent concentration.|
FunctionSeems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
Tissue specificityExpressed in the cerebral cortex and the cerebellum.
Involvement in diseaseNote=A chromosomal aberration involving SHANK3 is found in patients with chromosome 22q13.3 deletion syndrome. Translocation t(12;22)(q24.1;q13.3) with APPL2/DIP13B.
Note=Defects in SHANK3 are associated with autism spectrum disorders (ASD). ASD are characterized by impairments in reciprocal social interaction and communication as well as restricted and stereotyped patterns of interest and activities. ASD include forms with moderate to severe cognitive impairment and milder forms with higher cognitive ability (Asperger syndrome).
Defects in SHANK3 are the cause of schizophrenia type 15 (SCZD15) [MIM:613950]. SCZD15 is a complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder.
Sequence similaritiesContains 6 ANK repeats.
Contains 1 PDZ (DHR) domain.
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH3 domain.
Cellular localizationCytoplasm. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Postsynaptic density of neuronal cells.
- Information by UniProt
- AI841104 antibody
- DEL22q13.3 antibody
- KIAA1650 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab183420 has not yet been referenced specifically in any publications.