Overview

  • Product name

    Anti-SHARP1 antibody - N-terminal
    See all SHARP1 primary antibodies
  • Description

    Rabbit polyclonal to SHARP1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human SHARP1 aa 24-45 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. NP_110389.
    Database link: Q9C0J9

  • Positive control

    • RD cell lysate, NCI-H460 cells, MCF-7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab175544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000. Predicted molecular weight: 51 kDa.
ICC/IF 1/10 - 1/50.

Target

  • Function

    May be a transcriptional repressor that represses both basal and activated transcription.
  • Tissue specificity

    Highly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Basic helix-loop-helix domain containing class B3 antibody
    • BHE41_HUMAN antibody
    • bHLH protein DEC2 antibody
    • bHLHB3 antibody
    • bHLHe41 antibody
    • Class B basic helix loop helix protein 3 antibody
    • Class B basic helix-loop-helix protein 3 antibody
    • Class E basic helix-loop-helix protein 41 antibody
    • DEC2 antibody
    • Differentially expressed in chondrocytes protein 2 antibody
    • Enhancer of split and hairy related protein 1 antibody
    • Enhancer-of-split and hairy-related protein 1 antibody
    • hDEC2 antibody
    • Moderately similar to basic-helix loop helix protein M.musculus antibody
    • SHARP-1 antibody
    see all

Images

  • Anti-SHARP1 antibody - N-terminal (ab175544) at 1/1000 dilution + RD cell lysate at 35 µg

    Predicted band size: 51 kDa

  • Fluorescent confocal image of MCF-7 cell stained with ab175544 at 1/25 dilution.  MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab175544 (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SHARP1 immunoreactivity is localized to nucleus significantly.

  • Flow cytometric analysis of NCI-H460 cells (right histogram) labeling SHARP1 at 1/10 dilution compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:

  • Numata A  et al. The basic helix-loop-helix transcription factor SHARP1 is an oncogenic driver in MLL-AF6 acute myelogenous leukemia. Nat Commun 9:1622 (2018). Read more (PubMed: 29692408) »
See 1 Publication for this product

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