The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
1/5000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
Use at 1-4 µg/mg of lysate.
SHARP2 (enhancer–of-split and hairy-related protein, differentially expressed in chondrocytes protein 1, DEC1) is a class B basic helix-loop-helix protein.
Function: May function as a transcriptional factor for neuronal differentiation.
PTM: Ubiquitinated; which may lead to proteasomal degradation
Detection of Human SHARP2 by Immunoprecipitation in whole cell lysate from 293T cells (1 mg for IP, 20% of IP loaded), using ab70723 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of SHARP2 was performed using ab70723 at 1 µg/ml. Chemiluminescence with exposure time of 30 seconds