Key features and details
- Rabbit polyclonal to SHARP2/DEC1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SHARP2/DEC1 antibody
See all SHARP2/DEC1 primary antibodies
DescriptionRabbit polyclonal to SHARP2/DEC1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Zebrafish
Synthetic peptide corresponding to Human SHARP2/DEC1 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive control in the following human lysate: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate This antibody gave a positive control in the following mouse lysate: Liver tissue This antibody gave a positive control in the following rat lysate: Liver tissue
This product was previously labelled as SHARP2
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab23797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 46 kDa).|
FunctionTranscriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1
ARNTL2/BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity. May be involved in the regulation of chondrocyte differentiation via the cAMP pathway.
Tissue specificityExpressed in cartilage, spleen, intestine, lung, and to a lesser extent in heart, brain, liver, muscle and stomach.
Sequence similaritiesContains 1 bHLH (basic helix-loop-helix) domain.
Contains 1 Orange domain.
modificationsUbiquitinated; which may lead to proteasomal degradation.
Sumoylation inhibits its ubiquitination and promotes its negative regulation of the CLOCK-ARNTL/BMAL1 heterodimer transcriptional activator activity.
Cellular localizationCytoplasm. Nucleus. Predominantly localized in the nucleus (PubMed:11278694).
- Information by UniProt
- BHE40_HUMAN antibody
- bHLHB2 antibody
- bHLHe40 antibody
All lanes : Anti-SHARP2/DEC1 antibody (ab23797) at 1 µg
Lane 1 : Hela Whole Cell Lysate
Lane 2 :
Jurkat whole cell lysate (ab7899)
Lysates/proteins at 20 µg per lane.
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Predicted band size: 46 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa (possible non-specific binding), 37 kDa (possible cleavage fragment), 37 kDa (possible non-specific binding)
All lanes : Anti-SHARP2/DEC1 antibody (ab23797) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 2 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 33 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
ab23797 has been referenced in 1 publication.
- Vieira E et al. RELATIONSHIP BETWEEN AMPK AND THE TRANSCRIPTIONAL BALANCE OF CLOCK-RELATED GENES IN SKELETAL MUSCLE. Am J Physiol Endocrinol Metab : (2008). PubMed: 18728219