Anti-SHARP2/DEC1 antibody (ab70723)
Key features and details
- Rabbit polyclonal to SHARP2/DEC1
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SHARP2/DEC1 antibody
See all SHARP2/DEC1 primary antibodies -
Description
Rabbit polyclonal to SHARP2/DEC1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human SHARP2/DEC1 (C terminal).
Database link: NP_003661.1 -
Positive control
- Whole cell lysates from HeLa and 293T cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab70723 was affinity purified using an epitope specific to SHARP2/DEC1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
WB |
1/5000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
|
|
IP |
Use at 1-4 µg/mg of lysate.
|
Notes |
---|
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/5000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa). |
IP
Use at 1-4 µg/mg of lysate. |
Target
-
Function
Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1
ARNTL2/BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity. May be involved in the regulation of chondrocyte differentiation via the cAMP pathway. -
Tissue specificity
Expressed in cartilage, spleen, intestine, lung, and to a lesser extent in heart, brain, liver, muscle and stomach. -
Sequence similarities
Contains 1 bHLH (basic helix-loop-helix) domain.
Contains 1 Orange domain. -
Post-translational
modificationsUbiquitinated; which may lead to proteasomal degradation.
Sumoylation inhibits its ubiquitination and promotes its negative regulation of the CLOCK-ARNTL/BMAL1 heterodimer transcriptional activator activity. -
Cellular localization
Cytoplasm. Nucleus. Predominantly localized in the nucleus (PubMed:11278694). - Information by UniProt
-
Database links
- Entrez Gene: 8553 Human
- Omim: 604256 Human
- SwissProt: O14503 Human
- SwissProt: Q5RA17 Orangutan
- Unigene: 744856 Human
-
Alternative names
- BHE40_HUMAN antibody
- bHLHB2 antibody
- bHLHe40 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SHARP2/DEC1 with ab70723 at 1/1000 (1µg/ml). Detection: DAB.
-
Anti-SHARP2/DEC1 antibody (ab70723) at 0.1 µg/ml + Whole cell lysate from HeLa cells at 50 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Detection of Human SHARP2/DEC1 by Immunoprecipitation in whole cell lysate from 293T cells (1 mg for IP, 20% of IP loaded), using ab70723 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of SHARP2/DEC1 was performed using ab70723 at 1 µg/ml. Chemiluminescence with exposure time of 30 seconds
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab70723 has been referenced in 4 publications.
- Hu X et al. Overexpression of DEC1 in the epithelium of OSF promotes mesenchymal transition via activating FAK/Akt signal axis. J Oral Pathol Med 51:780-790 (2022). PubMed: 36053880
- Lv W et al. Differentiated embryonic chondrocyte-expressed gene 1 promotes temozolomide resistance by modulating the SP1-MGMT axis in glioblastoma. Am J Transl Res 13:2331-2349 (2021). PubMed: 34017393
- Mao T et al. DEC1: a potential biomarker of malignant transformation in oral leukoplakia. Braz Oral Res 34:e052 (2020). PubMed: 32578762
- Hwang JW et al. iPSC-Derived Embryoid Bodies as Models of c-Met-Mutated Hereditary Papillary Renal Cell Carcinoma. Int J Mol Sci 20:N/A (2019). PubMed: 31575031