Key features and details
- Rabbit polyclonal to SHARP2/DEC1
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SHARP2/DEC1 antibody
See all SHARP2/DEC1 primary antibodies
DescriptionRabbit polyclonal to SHARP2/DEC1
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human SHARP2/DEC1 (C terminal).
Database link: NP_003661.1
- Whole cell lysates from HeLa and 293T cells.
This product was previously labelled as SHARP2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70723 was affinity purified using an epitope specific to SHARP2/DEC1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab70723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/5000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).|
|IP||Use at 1-4 µg/mg of lysate.|
FunctionTranscriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1
ARNTL2/BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity. May be involved in the regulation of chondrocyte differentiation via the cAMP pathway.
Tissue specificityExpressed in cartilage, spleen, intestine, lung, and to a lesser extent in heart, brain, liver, muscle and stomach.
Sequence similaritiesContains 1 bHLH (basic helix-loop-helix) domain.
Contains 1 Orange domain.
modificationsUbiquitinated; which may lead to proteasomal degradation.
Sumoylation inhibits its ubiquitination and promotes its negative regulation of the CLOCK-ARNTL/BMAL1 heterodimer transcriptional activator activity.
Cellular localizationCytoplasm. Nucleus. Predominantly localized in the nucleus (PubMed:11278694).
- Information by UniProt
- BHE40_HUMAN antibody
- bHLHB2 antibody
- bHLHe40 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SHARP2/DEC1 with ab70723 at 1/1000 (1µg/ml). Detection: DAB.
Anti-SHARP2/DEC1 antibody (ab70723) at 0.1 µg/ml + Whole cell lysate from HeLa cells at 50 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Detection of Human SHARP2/DEC1 by Immunoprecipitation in whole cell lysate from 293T cells (1 mg for IP, 20% of IP loaded), using ab70723 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of SHARP2/DEC1 was performed using ab70723 at 1 µg/ml. Chemiluminescence with exposure time of 30 seconds
ab70723 has not yet been referenced specifically in any publications.