Overview

  • Product name

  • Description

    Rabbit polyclonal to SHARP2/DEC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human SHARP2/DEC1 (C terminal).
    Database link: NP_003661.1

  • Positive control

    • Whole cell lysates from HeLa and 293T cells.
  • General notes

     This product was previously labelled as SHARP2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab70723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
IP Use at 1-4 µg/mg of lysate.

Target

  • Function

    Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1
    ARNTL2/BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity. May be involved in the regulation of chondrocyte differentiation via the cAMP pathway.
  • Tissue specificity

    Expressed in cartilage, spleen, intestine, lung, and to a lesser extent in heart, brain, liver, muscle and stomach.
  • Sequence similarities

    Contains 1 bHLH (basic helix-loop-helix) domain.
    Contains 1 Orange domain.
  • Post-translational
    modifications

    Ubiquitinated; which may lead to proteasomal degradation.
    Sumoylation inhibits its ubiquitination and promotes its negative regulation of the CLOCK-ARNTL/BMAL1 heterodimer transcriptional activator activity.
  • Cellular localization

    Cytoplasm. Nucleus. Predominantly localized in the nucleus (PubMed:11278694).
  • Information by UniProt
  • Database links

  • Alternative names

    • BHE40_HUMAN antibody
    • bHLHB2 antibody
    • bHLHe40 antibody
    • Class B Basic Helix Loop Helix Protein 2 antibody
    • Class B basic helix-loop-helix protein 2 antibody
    • Class E basic helix loop helix protein 40 antibody
    • Class E basic helix-loop-helix protein 40 antibody
    • Clast5 antibody
    • DEC1 antibody
    • Differentially expressed in chondrocytes protein 1 antibody
    • E47 interaction protein 1 antibody
    • EIP1 antibody
    • Enhancer of split and hairy related protein 2 antibody
    • Enhancer-of-split and hairy-related protein 2 antibody
    • SHARP 2 antibody
    • SHARP-2 antibody
    • Stimulated by retinoic acid gene 13 protein antibody
    • STRA13 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SHARP2/DEC1 with ab70723 at 1/1000 (1µg/ml). Detection: DAB.

  • Anti-SHARP2/DEC1 antibody (ab70723) at 0.1 µg/ml + Whole cell lysate from HeLa cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 45 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • Detection of Human SHARP2/DEC1 by Immunoprecipitation in whole cell lysate from 293T cells (1 mg for IP, 20% of IP loaded), using ab70723 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of SHARP2/DEC1 was performed using ab70723 at 1 µg/ml. Chemiluminescence with exposure time of 30 seconds

References

ab70723 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab70723.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up