• Product name

  • Description

    Rabbit polyclonal to SHARP2/DEC1
  • Host species

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human SHARP2/DEC1 (C terminal).
    Database link: NP_003661.1

  • Positive control

    • Whole cell lysates from HeLa and 293T cells.
  • General notes

     This product was previously labelled as SHARP2




Our Abpromise guarantee covers the use of ab70723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
IP Use at 1-4 µg/mg of lysate.


  • Function

    Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1
    ARNTL2/BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity. May be involved in the regulation of chondrocyte differentiation via the cAMP pathway.
  • Tissue specificity

    Expressed in cartilage, spleen, intestine, lung, and to a lesser extent in heart, brain, liver, muscle and stomach.
  • Sequence similarities

    Contains 1 bHLH (basic helix-loop-helix) domain.
    Contains 1 Orange domain.
  • Post-translational

    Ubiquitinated; which may lead to proteasomal degradation.
    Sumoylation inhibits its ubiquitination and promotes its negative regulation of the CLOCK-ARNTL/BMAL1 heterodimer transcriptional activator activity.
  • Cellular localization

    Cytoplasm. Nucleus. Predominantly localized in the nucleus (PubMed:11278694).
  • Information by UniProt
  • Database links

  • Alternative names

    • BHE40_HUMAN antibody
    • bHLHB2 antibody
    • bHLHe40 antibody
    • Class B Basic Helix Loop Helix Protein 2 antibody
    • Class B basic helix-loop-helix protein 2 antibody
    • Class E basic helix loop helix protein 40 antibody
    • Class E basic helix-loop-helix protein 40 antibody
    • Clast5 antibody
    • DEC1 antibody
    • Differentially expressed in chondrocytes protein 1 antibody
    • E47 interaction protein 1 antibody
    • EIP1 antibody
    • Enhancer of split and hairy related protein 2 antibody
    • Enhancer-of-split and hairy-related protein 2 antibody
    • SHARP 2 antibody
    • SHARP-2 antibody
    • Stimulated by retinoic acid gene 13 protein antibody
    • STRA13 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SHARP2/DEC1 with ab70723 at 1/1000 (1µg/ml). Detection: DAB.

  • Anti-SHARP2/DEC1 antibody (ab70723) at 0.1 µg/ml + Whole cell lysate from HeLa cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 45 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 3 minutes
  • Detection of Human SHARP2/DEC1 by Immunoprecipitation in whole cell lysate from 293T cells (1 mg for IP, 20% of IP loaded), using ab70723 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of SHARP2/DEC1 was performed using ab70723 at 1 µg/ml. Chemiluminescence with exposure time of 30 seconds


ab70723 has not yet been referenced specifically in any publications.

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