Overview

  • Product name

  • Description

    Rabbit polyclonal to SHARPIN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Cow, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 25-75 of Human SHARPIN (NP_112236.3).

  • Positive control

    • HeLa, Jurkat and 293T whole cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 40 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    May have a role in normal immune development and control of inflammation.
  • Tissue specificity

    Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
  • Sequence similarities

    Contains 1 RanBP2-type zinc finger.
  • Cellular localization

    Cytoplasm. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp434N1923 antibody
    • hSIPL1 antibody
    • Shank associated RH domain interacting protein antibody
    • SHANK associated RH domain interactor antibody
    • Shank interacting protein like 1 antibody
    • Shank-associated RH domain-interacting protein antibody
    • Shank-interacting protein-like 1 antibody
    • Sharpin antibody
    • SHRPN_HUMAN antibody
    • SIPL1 antibody
    see all

Images

  • All lanes : Anti-SHARPIN antibody (ab125188) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 40 kDa


    Exposure time: 3 minutes
  • ab125188, at 6 µg/mg lysate, detecting SHARPIN in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
    For the subsequent Western blot, ab125188 was used at 1 µg/ml.
    The blot was developed using the ECL technique, with an exposure time of 30 seconds.

References

This product has been referenced in:

  • Füllsack S  et al. Redundant and receptor-specific activities of TRADD, RIPK1 and FADD in death receptor signaling. Cell Death Dis 10:122 (2019). Read more (PubMed: 30741924) »
  • Tang Y  et al. Linear ubiquitination of cFLIP induced by LUBAC contributes to TNFa-induced apoptosis. J Biol Chem 293:20062-20072 (2018). Read more (PubMed: 30361438) »
See all 4 Publications for this product

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