Key features and details
- Rabbit polyclonal to SHARPIN
- Suitable for: IP, WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SHARPIN antibody
See all SHARPIN primary antibodies
DescriptionRabbit polyclonal to SHARPIN
Tested applicationsSuitable for: IP, WB, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human SHARPIN aa 1-230 (N terminal).
MAPPAGGAAAAASDLGSAAVLLAVHAAVRPLGAGPDAEAQLRRLQLSADP ERPGRFRL ELLGAGPGAVNLEWPLESVSYTIRGPTQHELQPPPGGPGT LSLHFLNPQEAQRWAVLVR GATVEGQNGSKSNSPPALGPEACPVSLPS PPEASTLKGPPPEADLPRSPGNLTEREELAG SLARAIAGGDEKGAAQV AAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAAS
Database link: Q9H0F6
- Sharpin transfected U2OS cells and lysates, U2OS cell extracts, NIH3T3 cell lysate,
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab197853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||1/2000. Predicted molecular weight: 40 kDa.|
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay have a role in normal immune development and control of inflammation.
Tissue specificityHighly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
Sequence similaritiesContains 1 RanBP2-type zinc finger.
Cellular localizationCytoplasm. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1.
- Information by UniProt
- DKFZp434N1923 antibody
- hSIPL1 antibody
- Shank associated RH domain interacting protein antibody
All lanes : Anti-SHARPIN antibody (ab197853) at 1/2000 dilution
Lane 1 : Control siRNA transfected U2OS cell lysate
Lane 2 : Sharpin siRNA transfected U2OS cell lysate
Lane 3 : NIH3T3 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 40 kDa
Transfer in nitrocelullose, block: 1 hour 5% milk in TBST, incubation with primary antibody o/n @ 4oC in 5% milk TBST (0.1% Tween).
Western blot analysis with ab197853 of Immunoprecipitation
using U2OS cells extracts
Lane 1: Input.
Lane 2: Immunoprecipitation with control serum.
Lane 3: Immunoprecipitation with ab197853 (5μl)
Flow cytometric analysis of U2OS cells labeling Sharpin with ab197853 at 1/200 dilution. Upper panel shows labeling wit IgG controls, middle panel shows labeling of control siRNA treated cells and bottom panel shows labeling of Sharpin siRNA treated cells. Cells were fixed with 1% PFA. Cells were incubated with the primary antibody overnight at 4°C.
Immunofluorescent analysis of PFA fixed and methanol permeabilizaed U2OS cells transfected with HA-Sharpin labeling Sharpin with ab197853 at 1/200 dilution in PBST (green) and HA in (red)
ab197853 has been referenced in 3 publications.
- Yang Y et al. SHARPIN regulates cell proliferation of cutaneous basal cell carcinoma via inactivation of the transcriptional factors GLI2 and c-JUN. Mol Med Rep N/A:N/A (2020). WB ; Human . PubMed: 32319607
- Tang L et al. Down-regulated SHARPIN may accelerate the development of atopic dermatitis through activating interleukin-33/ST2 signalling. Exp Dermatol N/A:N/A (2018). WB ; Human . PubMed: 30230040
- Fu T et al. A novel SHARPIN-PRMT5-H3R2me1 axis is essential for lung cancer cell invasion. Oncotarget 8:54809-54820 (2017). WB ; Human . PubMed: 28903384