Product nameAnti-SHARPIN antibody
See all SHARPIN primary antibodies
DescriptionRabbit polyclonal to SHARPIN
Tested applicationsSuitable for: IP, WB, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human SHARPIN aa 1-230 (N terminal).
MAPPAGGAAAAASDLGSAAVLLAVHAAVRPLGAGPDAEAQLRRLQLSADP ERPGRFRL ELLGAGPGAVNLEWPLESVSYTIRGPTQHELQPPPGGPGT LSLHFLNPQEAQRWAVLVR GATVEGQNGSKSNSPPALGPEACPVSLPS PPEASTLKGPPPEADLPRSPGNLTEREELAG SLARAIAGGDEKGAAQV AAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAAS
Database link: Q9H0F6
- Sharpin transfected U2OS cells and lysates, U2OS cell extracts, NIH3T3 cell lysate,
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Our Abpromise guarantee covers the use of ab197853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||1/2000. Predicted molecular weight: 40 kDa.|
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay have a role in normal immune development and control of inflammation.
Tissue specificityHighly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
Sequence similaritiesContains 1 RanBP2-type zinc finger.
Cellular localizationCytoplasm. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1.
- Information by UniProt
- DKFZp434N1923 antibody
- hSIPL1 antibody
- Shank associated RH domain interacting protein antibody
All lanes : Anti-SHARPIN antibody (ab197853) at 1/2000 dilution
Lane 1 : Control siRNA transfected U2OS cell lysate
Lane 2 : Sharpin siRNA transfected U2OS cell lysate
Lane 3 : NIH3T3 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 40 kDa
Transfer in nitrocelullose, block: 1 hour 5% milk in TBST, incubation with primary antibody o/n @ 4oC in 5% milk TBST (0.1% Tween).
Western blot analysis with ab197853 of Immunoprecipitation
using U2OS cells extracts
Lane 1: Input.
Lane 2: Immunoprecipitation with control serum.
Lane 3: Immunoprecipitation with ab197853 (5μl)
Flow cytometric analysis of U2OS cells labeling Sharpin with ab197853 at 1/200 dilution. Upper panel shows labeling wit IgG controls, middle panel shows labeling of control siRNA treated cells and bottom panel shows labeling of Sharpin siRNA treated cells. Cells were fixed with 1% PFA. Cells were incubated with the primary antibody overnight at 4°C.
Immunofluorescent analysis of PFA fixed and methanol permeabilizaed U2OS cells transfected with HA-Sharpin labeling Sharpin with ab197853 at 1/200 dilution in PBST (green) and HA in (red)
This product has been referenced in:
- Tang L et al. Down-regulated SHARPIN may accelerate the development of atopic dermatitis through activating interleukin-33/ST2 signalling. Exp Dermatol N/A:N/A (2018). WB ; Human . Read more (PubMed: 30230040) »
- Fu T et al. A novel SHARPIN-PRMT5-H3R2me1 axis is essential for lung cancer cell invasion. Oncotarget 8:54809-54820 (2017). WB ; Human . Read more (PubMed: 28903384) »