Overview

  • Product name

  • Description

    Rabbit polyclonal to SHARPIN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment within Human SHARPIN (internal sequence). The exact sequence is proprietary.
    Database link: Q9H0F6

  • Positive control

    • IP: THP-1 whole cell extract. WB: K562 and THP-1 whole cell extracts.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
IP 1/100 - 1/800.

Target

  • Function

    May have a role in normal immune development and control of inflammation.
  • Tissue specificity

    Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
  • Sequence similarities

    Contains 1 RanBP2-type zinc finger.
  • Cellular localization

    Cytoplasm. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp434N1923 antibody
    • hSIPL1 antibody
    • Shank associated RH domain interacting protein antibody
    • SHANK associated RH domain interactor antibody
    • Shank interacting protein like 1 antibody
    • Shank-associated RH domain-interacting protein antibody
    • Shank-interacting protein-like 1 antibody
    • Sharpin antibody
    • SHRPN_HUMAN antibody
    • SIPL1 antibody
    see all

Images

  • SHARPIN was immunoprecipitated from THP-1 (human monocytic leukemia cell line) whole cell extracts using 5 μg ab227598. Western blot analysis was performed using ab227598. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Control IgG IP in THP-1 whole cell extract.

    Lane 2: ab227598 in THP-1 whole cell extract.

  • All lanes : Anti-SHARPIN antibody (ab227598) at 1/1000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract
    Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell extract

    Predicted band size: 40 kDa



    10% SDS-PAGE gel.

References

ab227598 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227598.
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