Key features and details
- Rabbit polyclonal to SHARPIN
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SHARPIN antibody
See all SHARPIN primary antibodies
DescriptionRabbit polyclonal to SHARPIN
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Recombinant fragment within Human SHARPIN (internal sequence). The exact sequence is proprietary.
Database link: Q9H0F6
- IP: THP-1 whole cell extract. WB: K562 and THP-1 whole cell extracts.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 40 kDa.|
|IP||1/100 - 1/800.|
FunctionMay have a role in normal immune development and control of inflammation.
Tissue specificityHighly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
Sequence similaritiesContains 1 RanBP2-type zinc finger.
Cellular localizationCytoplasm. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1.
- Information by UniProt
- DKFZp434N1923 antibody
- hSIPL1 antibody
- Shank associated RH domain interacting protein antibody
SHARPIN was immunoprecipitated from THP-1 (human monocytic leukemia cell line) whole cell extracts using 5 μg ab227598. Western blot analysis was performed using ab227598. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in THP-1 whole cell extract.
Lane 2: ab227598 in THP-1 whole cell extract.
All lanes : Anti-SHARPIN antibody (ab227598) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell extract
Predicted band size: 40 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227598 has not yet been referenced specifically in any publications.