Anti-SHARPIN antibody (ab236092)
Key features and details
- Rabbit polyclonal to SHARPIN
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SHARPIN antibody
See all SHARPIN primary antibodies -
Description
Rabbit polyclonal to SHARPIN -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human SHARPIN aa 50-200.
Database link: Q9H0F6 -
Positive control
- WB: HeLa, Jurkat, A549 and L02 whole cell lysates. IHC-P: Human skeletal muscle and prostate cancer tissue. ICC/IF: PC-3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Predicted molecular weight: 40 kDa.
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IHC-P |
1/500 - 1/1000.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/500 - 1/5000. Predicted molecular weight: 40 kDa. |
IHC-P
1/500 - 1/1000. |
ICC/IF
1/50 - 1/200. |
Target
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Function
May have a role in normal immune development and control of inflammation. -
Tissue specificity
Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. -
Sequence similarities
Contains 1 RanBP2-type zinc finger. -
Cellular localization
Cytoplasm. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1. - Information by UniProt
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Database links
- Entrez Gene: 81858 Human
- Omim: 611885 Human
- SwissProt: Q9H0F6 Human
- Unigene: 529755 Human
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Alternative names
- DKFZp434N1923 antibody
- hSIPL1 antibody
- Shank associated RH domain interacting protein antibody
see all
Images
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All lanes : Anti-SHARPIN antibody (ab236092) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 4 : L02 (human immortal liver cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40 kDa -
Paraffin-embedded human skeletal muscle tissue stained for SHARPIN using ab236092 at 1/500 dilution in immunohistochemical analysis. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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Paraffin-embedded human prostate cancer tissue stained for SHARPIN using ab236092 at 1/500 dilution in immunohistochemical analysis. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized PC-3 (human epithelial cell line from cervix adenocarcinoma) cells stained for SHARPIN (green) using ab236092 at 1/166 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236092 has not yet been referenced specifically in any publications.