Anti-SHARPIN antibody (ab69507)
Key features and details
- Mouse polyclonal to SHARPIN
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SHARPIN antibody
See all SHARPIN primary antibodies -
Description
Mouse polyclonal to SHARPIN -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human SHARPIN aa 1-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: AAH25244.1 -
Positive control
- SHARPIN transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 34 kDa. (predicted molecular weight: Isoform 2, 34 kDa; Isoform 1, 40 kDa).
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 34 kDa. (predicted molecular weight: Isoform 2, 34 kDa; Isoform 1, 40 kDa). |
Target
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Function
May have a role in normal immune development and control of inflammation. -
Tissue specificity
Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. -
Sequence similarities
Contains 1 RanBP2-type zinc finger. -
Cellular localization
Cytoplasm. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1. - Information by UniProt
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Database links
- Entrez Gene: 81858 Human
- Omim: 611885 Human
- SwissProt: Q9H0F6 Human
- Unigene: 529755 Human
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Alternative names
- DKFZp434N1923 antibody
- hSIPL1 antibody
- Shank associated RH domain interacting protein antibody
see all
Images
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All lanes : Anti-SHARPIN antibody (ab69507) at 1/500 dilution
Lane 1 : SHARPIN transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 34 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (8)
ab69507 has been referenced in 8 publications.
- Gao J et al. Sharpin suppresses ß1-integrin activation by complexing with the ß1 tail and kindlin-1. Cell Commun Signal 17:101 (2019). PubMed: 31429758
- Dorn S et al. LUBAC and ABIN-1 Modulate TRAIL-Based NF-?B Induction in Human Embryonic Kidney 293 Cells. Biores Open Access 7:81-89 (2018). PubMed: 29862142
- Khan MH et al. The Sharpin interactome reveals a role for Sharpin in lamellipodium formation via the Arp2/3 complex. J Cell Sci 130:3094-3107 (2017). PubMed: 28775156
- Yang H et al. SHARPIN Facilitates p53 Degradation in Breast Cancer Cells. Neoplasia 19:84-92 (2017). PubMed: 28063307
- Zhuang T et al. SHARPIN stabilizes estrogen receptor a and promotes breast cancer cell proliferation. Oncotarget 8:77137-77151 (2017). PubMed: 29100376
- De Franceschi N et al. Mutually Exclusive Roles of SHARPIN in Integrin Inactivation and NF-?B Signaling. PLoS One 10:e0143423 (2015). PubMed: 26600301
- Verhelst K et al. A20 inhibits LUBAC-mediated NF-?B activation by binding linear polyubiquitin chains via its zinc finger 7. EMBO J 31:3845-55 (2012). PubMed: 23032186
- Rantala JK et al. SHARPIN is an endogenous inhibitor of ß1-integrin activation. Nat Cell Biol 13:1315-24 (2011). PubMed: 21947080