Overview

  • Product name

  • Description

    Mouse polyclonal to SHARPIN
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human SHARPIN aa 1-326.
    Sequence:

    mappaggaaa aasdlgsaav llavhaavrp lgagpdaeaq lrrlqlsadp erpgrfrlel lgagpgavnl ewplesvsyt irgptqhelq pppggpgtls lhflnpqeaq rwavlvrgat vegqngsksn sppalgpeac pvslpsppea stlkgpppea dlprspgnlt ereelagsla raiaggdekg aaqvaavlaq hrvalsvqlq eacfppgpir lqvtledaas aasaassahv alqvhphctv aalqeqvfse lgfppavqrw vigrclcvpe rslasygvrq dgdpaflyll sapreapaql vlsflhlhqc prppwl


    Database link: AAH25244.1

  • Positive control

    • SHARPIN transfected 293T cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa. (predicted molecular weight: Isoform 2, 34 kDa; Isoform 1, 40 kDa).

Target

  • Function

    May have a role in normal immune development and control of inflammation.
  • Tissue specificity

    Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
  • Sequence similarities

    Contains 1 RanBP2-type zinc finger.
  • Cellular localization

    Cytoplasm. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp434N1923 antibody
    • hSIPL1 antibody
    • Shank associated RH domain interacting protein antibody
    • SHANK associated RH domain interactor antibody
    • Shank interacting protein like 1 antibody
    • Shank-associated RH domain-interacting protein antibody
    • Shank-interacting protein-like 1 antibody
    • Sharpin antibody
    • SHRPN_HUMAN antibody
    • SIPL1 antibody
    see all

Images

  • All lanes : Anti-SHARPIN antibody (ab69507) at 1/500 dilution

    Lane 1 : SHARPIN transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 34 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Dorn S  et al. LUBAC and ABIN-1 Modulate TRAIL-Based NF-?B Induction in Human Embryonic Kidney 293 Cells. Biores Open Access 7:81-89 (2018). Read more (PubMed: 29862142) »
  • Khan MH  et al. The Sharpin interactome reveals a role for Sharpin in lamellipodium formation via the Arp2/3 complex. J Cell Sci 130:3094-3107 (2017). Read more (PubMed: 28775156) »
See all 7 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab69507.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up