Overview

  • Product name
    Anti-SHARPIN antibody
    See all SHARPIN primary antibodies
  • Description
    Mouse polyclonal to SHARPIN
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human SHARPIN protein (AAH25244.1, 1 a.a. ~ 326 a.a)

  • Positive control
    • SHARPIN transfected 293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa. (predicted molecular weight: Isoform 2, 34 kDa; Isoform 1, 40 kDa).

Target

  • Function
    May have a role in normal immune development and control of inflammation.
  • Tissue specificity
    Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
  • Sequence similarities
    Contains 1 RanBP2-type zinc finger.
  • Cellular localization
    Cytoplasm. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1.
  • Information by UniProt
  • Database links
    • Alternative names
      • DKFZp434N1923 antibody
      • hSIPL1 antibody
      • Shank associated RH domain interacting protein antibody
      • SHANK associated RH domain interactor antibody
      • Shank interacting protein like 1 antibody
      • Shank-associated RH domain-interacting protein antibody
      • Shank-interacting protein-like 1 antibody
      • Sharpin antibody
      • SHRPN_HUMAN antibody
      • SIPL1 antibody
      see all

    Images

    • Western blot - Anti-SHARPIN antibody (ab69507)
      Western blot - Anti-SHARPIN antibody (ab69507)
      All lanes : Anti-SHARPIN antibody (ab69507) at 1/500 dilution

      Lane 1 : SHARPIN transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size: 34 kDa
      Observed band size: 36 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:

    See all 6 Publications for this product

    Publishing research using ab69507? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab69507.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up