Overview

  • Product name

    Anti-SHB antibody [EPR7976]
    See all SHB primary antibodies
  • Description

    Rabbit monoclonal [EPR7976] to SHB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SHB aa 450-550. The exact sequence is proprietary.

  • Positive control

    • HepG2 and K562 cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic-FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Contains 1 SH2 domain.
    • Domain

      The SH2 domain preferentially binds phosphopeptides with the consensus sequence Y-[TVI]-X-L and mediates interaction with PDGFRA, PDGFRB, FGRFR1, IL2RB, IL2RG, CD3Z and CRK/CrKII.
    • Post-translational
      modifications

      Phosphorylated upon PDGFRA, PDGFRB, TCR, IL2 receptor, FGFR1 or VEGFR2 activation.
    • Cellular localization

      Cytoplasm. Cell membrane. Associates with membrane lipid rafts upon TCR stimulation.
    • Information by UniProt
    • Database links

    • Alternative names

      • bA3J10.2 antibody
      • OTTHUMP00000021397 antibody
      • RP11 3J10.8 antibody
      • RP113J10.8 antibody
      • SH2 domain containing adapter protein B antibody
      • SH2 domain-containing adapter protein B antibody
      • SHB (Src homology 2 domain containing) adaptor protein B antibody
      • SHB adaptor protein (a Src homology 2 protein) antibody
      • SHB adaptor protein antibody
      • Shb antibody
      • SHB_HUMAN antibody
      • Src homology 2 domain containing adaptor protein B antibody
      • Src homology 2 protein antibody
      see all

    Images

    • All lanes : Anti-SHB antibody [EPR7976] (ab129190) at 1/1000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : K562 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 55 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for your response.

    The epitope has not been mapped yet, so this information needs to be determined.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Answer

    I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

    In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.

    As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

    All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.

    Could you please provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.

    Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

    Read More

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