Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7976] to SHB
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-SHB antibody [EPR7976]
See all SHB primary antibodies
DescriptionRabbit monoclonal [EPR7976] to SHB
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SHB aa 450-550. The exact sequence is proprietary.
- HepG2 and K562 cell lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.21 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 55 kDa.|
FunctionAdapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic-FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells.
Tissue specificityWidely expressed.
Sequence similaritiesContains 1 SH2 domain.
DomainThe SH2 domain preferentially binds phosphopeptides with the consensus sequence Y-[TVI]-X-L and mediates interaction with PDGFRA, PDGFRB, FGRFR1, IL2RB, IL2RG, CD3Z and CRK/CrKII.
modificationsPhosphorylated upon PDGFRA, PDGFRB, TCR, IL2 receptor, FGFR1 or VEGFR2 activation.
Cellular localizationCytoplasm. Cell membrane. Associates with membrane lipid rafts upon TCR stimulation.
- Information by UniProt
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All lanes : Anti-SHB antibody [EPR7976] (ab129190) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : K562 cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 55 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab129190 has been referenced in 1 publication.
- Vanli G et al. Evaluation and validation of commercial antibodies for the detection of Shb. PLoS One 12:e0188311 (2017). PubMed: 29194461