RecombinantRabMAb

Recombinant Anti-SHC (phospho Y427) antibody [EPR778(2)Y] (ab68166)

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Western blot - Anti-SHC (phospho Y427) antibody [EPR778(2)Y] (ab68166)
  • Anti-SHC (phospho Y427) antibody [EPR778(2)Y] (ab68166)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR778(2)Y] to SHC (phospho Y427)
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-SHC (phospho Y427) antibody [EPR778(2)Y]
    See all SHC primary antibodies

  • Description

    Rabbit monoclonal [EPR778(2)Y] to SHC (phospho Y427)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC-Fr,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    A phospho specific peptide corresponding to residues surrounding tyrosine 427 of Human SHC.

  • Positive control

    • A431 cells treated with EGF

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab68166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 46, 52, 66 kDa (predicted molecular weight: 63 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,IHC-Fr,IHC-P or IP.

    • Target

    • Function

      Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.

    • Tissue specificity

      Widely expressed. Expressed in neural stem cells but absent in mature neurons.

    • Sequence similarities

      Contains 1 PID domain.
      Contains 1 SH2 domain.

    • Domain

      In response to a variety of growth factors, isoform p46Shc and isoform p52Shc bind to phosphorylated Trk receptors through their phosphotyrosine binding (PID) and/or SH2 domains. The PID and SH2 domains bind to specific phosphorylated tyrosine residues in the Asn-Pro-Xaa-Tyr(P) motif of the Trk receptors. Isoform p46Shc and isoform p52Shc are in turn phosphorylated on three tyrosine residues within the extended proline-rich domain. These phosphotyrosines act as docking site for GRB2 and thereby are involved in Ras activation.

    • Post-translational
      modifications

      Phosphorylated by activated epidermal growth factor receptor. Phosphorylated in response to FLT4 and KIT signaling. Isoform p46Shc and isoform p52Shc are phosphorylated on tyrosine residues of the Pro-rich domain. Isoform p66Shc is phosphorylated on Ser-36 by PRKCB upon treatment with insulin, hydrogen peroxide or irradiation with ultraviolet light (By similarity). Tyrosine phosphorylated in response to FLT3 signaling (By similarity). Tyrosine phosphorylated by activated PTK2B/PYK2 (By similarity). Tyrosine phosphorylated by ligand-activated ALK. Tyrosine phosphorylated by ligand-activated PDGFRB. Tyrosine phosphorylated by TEK/TIE2. May be tyrosine phosphorylated by activated PTK2/FAK1; tyrosine phosphorylation was seen in an astrocytoma biopsy, where PTK2/FAK1 kinase activity is high, but not in normal brain tissue. Isoform p52Shc dephosphorylation by PTPN2 may regulate interaction with GRB2.

    • Cellular localization

      Cytoplasm; Mitochondrion matrix. Localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization and Mitochondrion. In case of oxidative conditions, phosphorylation at 'Ser-36' of isoform p66Shc, leads to mitochondrial accumulation.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • FLJ26504 antibody
      • p66 antibody
      • p66SHC antibody
      • SH2 domain protein C1 antibody
      • SHC (Src homology 2 domain containing) transforming protein 1 antibody
      • SHC 1 antibody
      • SHC A antibody
      • SHC adaptor protein 1 antibody
      • Shc antibody
      • SHC transforming protein 1 antibody
      • SHC transforming protein antibody
      • SHC-transforming protein 1 antibody
      • SHC-transforming protein 3 antibody
      • SHC-transforming protein A antibody
      • SHC1 antibody
      • SHC1_HUMAN antibody
      • SHCA antibody
      • Src homology 2 domain-containing-transforming protein C1 antibody
      see all

    Images

    • Western blot - Anti-SHC (phospho Y427) antibody [EPR778(2)Y] (ab68166)
      Western blot - Anti-SHC (phospho Y427) antibody [EPR778(2)Y] (ab68166)
      All lanes : Anti-SHC (phospho Y427) antibody [EPR778(2)Y] (ab68166) at 1/500 dilution

      Lane 1 : A431 lysate
      Lane 2 : A431 lysate - EGF treated

      Lysates/proteins at 10 µg/ml per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 63 kDa
      Observed band size: 66 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 46 kDa (possible isoform), 52 kDa (possible isoform)



      Beta tubulin loading control is also shown
    • Anti-SHC (phospho Y427) antibody [EPR778(2)Y] (ab68166)
      Anti-SHC (phospho Y427) antibody [EPR778(2)Y] (ab68166)

    References (0)

    Publishing research using ab68166? Please let us know so that we can cite the reference in this datasheet.

    ab68166 has not yet been referenced specifically in any publications.

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