Activates both Ras-dependent and Ras-independent migratory pathways in melanomas. Contributes to the early phases of agrin-induced tyrosine phosphorylation of CHRNB1.
Only expressed in melanomas. Weakly expressed in normal melanocytes and benign nevi. Highly expressed at the transition from radial growth phase to vertical growth phase and metastatic melanomas, when tumor cells acquire migratory competence and invasive potential.
Contains 1 PID domain. Contains 1 SH2 domain.
Phosphorylated; the phosphorylation is enhanced by EGF. Phosphorylation at Tyr-424 is required for the interaction with GRB2.
Cell junction > synapse > postsynaptic cell membrane. Colocalized with MUSK at the neuromuscular junction.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling SHC4 with ab174908 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.