Overview

  • Product name

    Anti-SHCBP1 antibody [EPR18843]
    See all SHCBP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR18843] to SHCBP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Mouse SHCBP1 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Z179

  • Positive control

    • WB: NIH/3T3 treated with 0.5% FBS for 48 hours then changed to 20% FBS for 28 hours whole cell lysate; Mouse embryo lysate; P0 mouse brain lysate; Rat embryo lysate; P0 rat brain lysate; Mouse thymus and lung lysates; Human fetal heart and fetal kidney lysates; HeLa, A431, Jurkat, Neuro-2a, C6, PC-12, RAW 264.7 and NIH/3T3 whole cell lysates. IP: Mouse thymus whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18843
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
IP 1/40.

Target

Images

  • All lanes : Anti-SHCBP1 antibody [EPR18843] (ab184467) at 1/5000 dilution

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) treated with 0.5% FBS for 48 hours whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) treated with 0.5% FBS for 48 hours then changed to 20% FBS for 28 hours whole cell lysate
    Lane 3 : Mouse embryo lysate
    Lane 4 : P0 mouse brain lysate
    Lane 5 : Rat embryo lysate
    Lane 6 : P0 rat brain lysate
    Lane 7 : Mouse liver lysate
    Lane 8 : Mouse thymus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 76 kDa
    Observed band size: 76 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1, 2, 3, 4, 5 and 6: 3 minutes; Lane 7 and 8: 30 seconds.

    SHCBP1 plays important roles in embryonic development, cytokinesis, neuronal development, tumorigenesis and T cell development. It is highly expressed in thymus but absent in liver. It can be induced in serum-stimulated NIH/3T3 cells. (PMID: 10086341 and 25153088).

  • All lanes : Anti-SHCBP1 antibody [EPR18843] (ab184467) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 76 kDa
    Observed band size: 76 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 30 seconds; Lane 2: 8 seconds.

  • All lanes : Anti-SHCBP1 antibody [EPR18843] (ab184467) at 1/5000 dilution

    Lane 1 : Mouse lung lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 5 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 76 kDa
    Observed band size: 76 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposuretimes: Lane 1, 2, 3 and 4: 30 seconds; Lane 5: 8 seconds.

  • All lanes : Anti-SHCBP1 antibody [EPR18843] (ab184467) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 76 kDa
    Observed band size: 76 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • SHCBP1 was immunoprecipitated from 0.35 mg of Mouse thymus whole cell lysate with ab184467 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184467 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse thymus whole cell lysate, 10µg (Input).

    Lane 2: ab184467 IP in Mouse thymus whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184467 in Mouse thymus whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

References

This product has been referenced in:

See 1 Publication for this product

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