Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane, cytoplasm and cell junctions. Recommended concentration of ab122310 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling SHCBP1L with ab122310. Staining shows strong cytoplasmic positivity in cells in seminiferous ducts.
Western blot - Anti-SHCBP1L antibody (ab122310)
All lanes : Anti-SHCBP1L antibody (ab122310) at 1/250 dilution
Lane 1 : RT-4 cell lysate Lane 2 : U-251 MG cell lysate Lane 3 : Human plasma Lane 4 : Human Liver lysate Lane 5 : Human Tonsil lysate