Overview

  • Product name

    Sheep Anti-Cow IgG1 H&L (HRP)
    See all IgG1 secondary antibodies
  • Description

    Sheep polyclonal Secondary Antibody to Cow IgG1 (HRP)
  • Host species

    Sheep
  • Target species

    Cow
  • Specificity

    By immunoelectrophoresis and ELISA ab112754 reacts specifically with bovine IgG1. This antibody may cross react with IgG1 from other species.
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Proclin
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure subclass specificity. ab112754 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
  • Conjugation notes

    Molar enzyme/antibody protein ratio is 4:1.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. with colorimetric detection. Use at 1/1000 - 1/30000 with chemiluminescent detection.
ELISA 1/10000 - 1/100000. as primary antibody.
IHC-P 1/200 - 1/500.
ICC/IF 1/200 - 1/500.

References

This product has been referenced in:

  • Araujo IL  et al. Immune responses in bovines to recombinant glycoprotein D of bovine herpesvirus type 5 as vaccine antigen. Vaccine 36:7708-7714 (2018). Read more (PubMed: 30381153) »
See 1 Publication for this product

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