Product nameSheep F(ab) Anti-Guinea pig IgG H&L (HRP)
See all IgG secondary antibodies
Target speciesGuinea pig
Tested applicationsSuitable for: WB, IHC-Fr, IHC-P, ICC/IF, ELISAmore details
Full length native protein (purified) corresponding to Guinea pig IgG.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituents: 49% Tris buffered saline, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesVery stringent purification protocol.
General notesDo not store in plastic tubes (i.e. eppendorf) to avoid losing activity
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Our Abpromise guarantee covers the use of ab136791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. 4CN (4-chloro-1naphtol): 25mg in 49.5ml of 0.1M pH 7.4 PBS + 5µl/ml 100% ethanol. Filter on filter paper. Add 25µl of 30% H2O2. Incubate 10 min at room temperature.|
|IHC-Fr||1/20 - 1/100. DAB (diamino-benzidine): 0.50mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.|
|IHC-P||1/20 - 1/100. DAB (diamino-benzidine): 0.50mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.|
|ICC/IF||1/20 - 1/100.|
|ELISA||1/500 - 1/5000. OPD (orthophenylene diamine): 0.4mg/ml in a 0.1M pH5 phosphate citrate buffer + 0.4µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness. Stop reaction with V/2 volume of 1M H2SO4. Reading at 492nm|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab136791 has not yet been referenced specifically in any publications.