Sheep IgG - Isotype Control (ab37385)

Q&A (4)References (3)

Overview

  • Product name

    Sheep IgG - Isotype Control
    See all Sheep isotype controls

  • Specificity

    This antibody has been selected to be an isotype control as it has no known specificity.

  • Tested applications

    Suitable for: ELISA, Flow Cytmore details

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Purified from normal sheep serum.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

  • Alternative names

    • Sheep Isotype Control

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab37385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent dilution.
Flow Cyt
Use 1µg for 106 cells.
Notes
ELISA
Use at an assay dependent dilution.
Flow Cyt
Use 1µg for 106 cells.

References (3)

Publishing research using ab37385? Please let us know so that we can cite the reference in this datasheet.

ab37385 has been referenced in 3 publications.

  • Luo RY  et al. ProBDNF promotes sepsis-associated encephalopathy in mice by dampening the immune activity of meningeal CD4+ T cells. J Neuroinflammation 17:169 (2020). PubMed: 32466783
  • Perera ON  et al. Telomerase promotes formation of a telomere protective complex in cancer cells. Sci Adv 5:eaav4409 (2019). PubMed: 31616780
  • Celik H  et al. JARID2 Functions as a Tumor Suppressor in Myeloid Neoplasms by Repressing Self-Renewal in Hematopoietic Progenitor Cells. Cancer Cell 34:741-756.e8 (2018). PubMed: 30423295

Customer reviews and Q&As

Q&A
Submit a question

1-4 of 4 Q&A

Question

Do you have FITC -conjugate sheep IgG for an isotype control?

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Answer

Following up my message from yesterday, we have added FITC-conjugated sheep IgG to our catalogue: ab127762.

If you have any questions, please contact us.

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Question

Do you have FITC -conjugated sheep IgG for an isotype control?

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Answer

Thank you for contacting us.

I am looking into adding a sheep isotype control FITC conjugate to our catalogue. I will have an answer regarding avaiaibility tomorrow.

Currently, what we do have is unconjugated IgG (ab37385) and a conjugation kit for creating a direct conjugate. The FITC kit comes in three sizes:

Click here (or use the following: https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-1-x-1mg-FITC-ab102883.html).

Click here (or use the following: https://www.abcam.com/FITC-Conjugation-Kit-Lightning-Linkreg-ab102884.html).

Click here (or use the following: https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-3-x-10g-FITC-ab102885.html).

But I understand you would prefer a ready-to-use product and will do what I can to obtain one for you.

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Question

A customer has the following enquiry;

On the data sheet, it says that these two antibodies should be stored at -20'C which is what I have done. Interestingly ab1893 does not seem to freeze and is still in a liquid state. Is this normal? Will ab37385 freeze too?

ab1893

Lot #: GR10346-10
Abcam invoice number: 1294594B



AB37385
Lot #: GR64701

Abcam invoice number 1308185



Many thanks for your time!

Kind regards,

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Answer

Thank you for contacting us.

We have checked the instock vials and can confirm that the liquid is frozen at -20C. You may need to check the freezer temperature because the antibody buffer should get frozen at -20C.

There is also no glycerol in storage buffer.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Does this product contain sodium azide? Has an endotoxin assay test been performed on this product?

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Answer

Thank you for your enquiry. I can confirm that this product does not contain sodium azide. It is supplied in Borate Buffered Saline, pH 8.0. This product has not been tested for endotoxin levels. Please let me know if you have any further questions.

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