Overview

  • Product name

    Sheep Anti-Mouse IgG H&L (beta-galactosidase)
    See all IgG secondary antibodies
  • Host species

    Sheep
  • Target species

    Mouse
  • Specificity

    Beta-galactosidase labelling provides good sensitivity and does not present endogenous activity in mammalian cells. It is well adapted to ELISA and produces no or very little background. It is more difficult to use in immunochemistry where its use can be justified only when the endogenous activities of other available labels produce significant problems.
  • Tested applications

    Suitable for: ICC, ELISA, IHC-P, IHC-Fr, WBmore details
  • Immunogen

    Highly purified whole immunoglobulin from mouse.

  • Conjugation

    beta-galactosidase

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituents: 50% Glycerol, 50% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab136776 has been purified using a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Preparation of Substrate Solutions: 0.1M phosphate buffer pH7, 1mM MgSO4, 0.2mM MnSO4, 2mM Mg titriplex. Add extemporaneously 7µl/ml beta-mercaptoethanol and 0.9mg/ml ortho-nitrophenyl-beta-galactopyranoside. Incubate for 30 minutes at 37°C. Stop the reaction with V/2 volume of 1M Na2CO3.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
ELISA 1/100 - 1/1000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

References

ab136776 has not yet been referenced specifically in any publications.

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