Sheep Anti-Mouse IgG H&L (Biotin) (ab6807)
Key features and details
- Sheep Anti-Mouse IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Sheep
- Suitable for: Immunomicroscopy, WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr
Related conjugates and formulations
Overview
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Product name
Sheep Anti-Mouse IgG H&L (Biotin)
See all IgG secondary antibodies -
Host species
Sheep -
Target species
Mouse -
Tested applications
Suitable for: Immunomicroscopy, WB, ICC/IF, Dot blot, ELISA, IHC-P, IHC-Frmore details -
Immunogen
Mouse IgG whole molecule
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule. -
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Immunomicroscopy |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent dilution.
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ICC/IF |
1/1000 - 1/2000.
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Dot blot |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent dilution.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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Immunomicroscopy
Use at an assay dependent concentration. |
WB
Use at an assay dependent dilution. |
ICC/IF
1/1000 - 1/2000. |
Dot blot
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent dilution. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab6807 has been referenced in 3 publications.
- Manickam R et al. Nampt activator P7C3 ameliorates diabetes and improves skeletal muscle function modulating cell metabolism and lipid mediators. J Cachexia Sarcopenia Muscle 13:1177-1196 (2022). PubMed: 35060352
- Rao VV & Mohanty A Immunohistochemical Identification of Muscle Fiber Types in Mice Tibialis Anterior Sections. Bio Protoc 9:e3400 (2019). PubMed: 33654901
- Xu W et al. FOXD3 Suppresses Tumor-Initiating Features in Lung Cancer via Transcriptional Repression of WDR5. Stem Cells 37:582-592 (2019). PubMed: 30703266