Overview

  • Product name
    Sheep Anti-Mouse IgG H&L (FITC)
    See all IgG secondary antibodies
  • Host species
    Sheep
  • Target species
    Mouse
  • Tested applications
    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    Mouse IgG whole molecule

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles DTAF per mole of Rabbit IgG
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
Flow Cyt
ICC/IF
IHC-P
IHC-Fr
ELISA
  • Application notes
    suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
  • References

    ab6804 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Answer

    Thank you for getting back to me and sharing your results.

    I am very pleased to hear that the new secondary antibody has worked well. This does indeed point to there having been a problem with the secondary antibody that you purchased from us as well as the replacement I sent you. I am not sure of the reason for this but I will be looking into it in more detail. I am very sorry for the inconvenience caused by these problems.

    I can arrange for a refund directly for you of the ab6804 if this is what you would like. This will be refunded directly to the account which paid for the original order.Alternatively, I can arrange for any antibody of your choice to be sent instead or raise a credit note which can be used for future purchases.

    Please let me know how you would like to proceed and I will arrange it for you.

    Read More

    Question
    Answer

    Thank you for getting back to me. I am sorry for my late reply.

    I am really not sure where these experiments may be going wrong, with so many different conditions tried I really cannot explain why no staining has been observed in every case.I think I would try to find out a few fundamental things to try and rule outwhat may be contributing to the problems:

    1. Have you performed any other staining with a FITC conjugated secondary antibody which has worked? I would check that both of the secondary antibodies are working as expected by putting a little sample directly on a slide and taking an image.

    2. Do you have any anti-mouse IgG which you know have been working well with any other primary antibodies? Perhaps with a different conjugate? Could you try initially just to see if the two primary antibodies are detecting the expected protein by using this alternative secondary antibody?

    3. You mentioned that co-staining with Alexafluor has worked well. What primary and secondary antibodywere used for this staining? Which antibodies were applied in what order when the dual staining was performed?

    I look forward to receiving your reply.

    Read More

    Answer

    Thank you for getting back to me.

    I am sorry to hear that the new secondary antibody has not made a difference in the results obtained. I would be happy to try to help in understanding why this might be happening.I think it would be useful if you could maybe fill in the questionnaire I have attached to this email. This information will allow me to see if there are any suggestions I would make in altering the protocol used.

    I think reducing the fixation time and using fresh PFA is a good start and I'll let you know if I have any further suggestions once I have received the completed questionnaire.

    I look forward to receiving your reply.

    Read More

    Answer

    Thank you for confirming that for me.

    I have arranged for the replacement to be sent to you. This is on the order number xxxxx(Purchase order number FOCR xxxxxx). This should be with you tomorrow. Please let me know if you have any problems receiving it.

    If you continue to have problems in the staining please do also let me know.

    Until then, I wish you all the best with your experiments.

    Read More

    Answer

    Thank you for confirming that.

    I can send you a new secondary antibody to try if you would like. I would suggest the following would be suitable:

    Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (FITC) (ab97239)

    Sheep polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab102457)

    If you let me know which one you would like to try (or a different one altogether if you prefer). I can then arrange for it to be sent to you, free of charge.

    I look forward to receiving your reply.

    Read More

    Answer

    Thank you for getting back to me with that information.

    I am sorry but I am a little confused with what has been tested? Which alternative antibody was tried with the secondary antibody which again did not work? On the orderof the 16th of July I only have the ab18058 and ab6804. Do you know the catalogue number of the antibody used?

    I will try to resolve this situation as soon as possible. I am sorry for the inconvenience this problem has been causing you.

    I look forward to receiving your reply.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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