Sheep Anti-Mouse IgG H&L (HRP) (ab6808)

Submit a review Q&A (1)References (38)
Western blot - Sheep Anti-Mouse IgG H&L (HRP) (ab6808)

    Key features and details

    • Sheep Anti-Mouse IgG H&L (HRP)
    • Conjugation: HRP
    • Host species: Sheep
    • Isotype: IgG
    • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

    Conjugates logo Related conjugates and formulations

    Biotin FITC Texas Red ® TRITC Unconjugated

    Overview

    • Product name

      Sheep Anti-Mouse IgG H&L (HRP)
      See all IgG secondary antibodies

    • Host species

      Sheep

    • Target species

      Mouse

    • Tested applications

      Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

    • Immunogen

      Mouse IgG whole molecule

    • Conjugation

      HRP

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      Preservative: 0.01% Gentamicin sulphate
      Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
    • Concentration information loading...

    • Purity

      Affinity purified

    • Purification notes

      Sheep Anti-Mouse IgG H&L was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6808 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    1/500 - 1/2500.
    Dot blot
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent dilution.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    Immunomicroscopy
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent dilution.
    Notes
    ICC/IF
    1/500 - 1/2500.
    Dot blot
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent dilution.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    Immunomicroscopy
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent dilution.

    Images

    • Western blot - Sheep Anti-Mouse IgG H&L (HRP) (ab6808)
      Western blot - Sheep Anti-Mouse IgG H&L (HRP) (ab6808)

      ab6808 was used at dilution 1/5000 with the primary antibody ab55120 in WB. See the review on ab55120.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (38)

    Publishing research using ab6808? Please let us know so that we can cite the reference in this datasheet.

    ab6808 has been referenced in 38 publications.

    • Hou Y  et al. Oncogenic miR-27a delivered by exosomes binds to SFRP1 and promotes angiogenesis in renal clear cell carcinoma. Mol Ther Nucleic Acids 24:92-103 (2021). PubMed: 33738141
    • Cowman S  et al. Downregulation of both mismatch repair and non-homologous end-joining pathways in hypoxic brain tumour cell lines. PeerJ 9:e11275 (2021). PubMed: 33986995
    • Begovich K  et al. Conserved metabolite regulation of stress granule assembly via AdoMet. J Cell Biol 219:N/A (2020). PubMed: 32609300
    • Wu JT  et al. Potential synergistic effects of sorafenib and CP-31398 for treating anaplastic thyroid cancer with p53 mutations. Oncol Lett 19:3021-3026 (2020). PubMed: 32218859
    • Liang YJ  et al. Statin inhibits large hepatitis delta antigen-Smad3 -twist-mediated epithelial-to-mesenchymal transition and hepatitis D virus secretion. J Biomed Sci 27:65 (2020). PubMed: 32434501
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I am looking for a secondary antibody, a anti-mouse which is HRP conjugated. It has to be rat absorbed and suitable for Western blots. Can you help? Thankyou Rebecca Upton NHLI London

    Read More
    Answer

    We do not have such an antibody available at present, however we have sourced some and these will be added to our website catalogue by 25th May. You should be able to find them by browsing the secondary antibodies section under anti-mouse (choose from the list of conjugates to narrow the search). The product codes will be ab7061 and ab7068 (raised in donkey and goat, respectively).

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