Sheep Anti-Mouse IgG H&L (HRP) (ab6808)

Submit a review Q&A (1)References (24)

Overview

  • Product name

    Sheep Anti-Mouse IgG H&L (HRP)
    See all IgG secondary antibodies

  • Host species

    Sheep

  • Target species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

  • Immunogen

    Mouse IgG whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Horseradish Peroxidase (HRP).

  • Conjugation notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB Use at an assay dependent dilution.

Images

  • Western blot - Sheep Anti-Mouse IgG H&L (HRP) (ab6808)
    Western blot - Sheep Anti-Mouse IgG H&L (HRP) (ab6808)
    Ab6808 was used at dilution 1/5000 with the primary antibody ab55120 in WB. See the review on ab55120.

References

This product has been referenced in:

  • Cowman S  et al. Decrease of Nibrin expression in chronic hypoxia is associated with hypoxia-induced chemoresistance in some brain tumour cells. BMC Cancer 19:300 (2019). Read more (PubMed: 30943920) »
  • Santini CC  et al. Global view of the RAF-MEK-ERK module and its immediate downstream effectors. Sci Rep 9:10865 (2019). Read more (PubMed: 31350469) »
See all 24 Publications for this product

Publishing research using ab6808? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

I am looking for a secondary antibody, a anti-mouse which is HRP conjugated. It has to be rat absorbed and suitable for Western blots. Can you help? Thankyou Rebecca Upton NHLI London

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Answer

We do not have such an antibody available at present, however we have sourced some and these will be added to our website catalogue by 25th May. You should be able to find them by browsing the secondary antibodies section under anti-mouse (choose from the list of conjugates to narrow the search). The product codes will be ab7061 and ab7068 (raised in donkey and goat, respectively).

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