Sheep Anti-Mouse IgG H&L (HRP) (ab6808)

Submit a review Q&A (1)References (19)

Overview

  • Product name
    Sheep Anti-Mouse IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species
    Sheep
  • Target species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    Mouse IgG whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Horseradish Peroxidase (HRP).
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB Use at an assay dependent dilution.

Images

  • Western blot - Sheep polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6808)
    Western blot - Sheep polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6808)
    Ab6808 was used at dilution 1/5000 with the primary antibody ab55120 in WB. See the review on ab55120.

References

This product has been referenced in:
  • Zhu G  et al. Expression of TRPC5 is decreased in the sperm of patients with varicocele-associated asthenozoospermia. Biomed Rep 8:529-534 (2018). Read more (PubMed: 29774143) »
  • Lu TY  et al. Axon degeneration induces glial responses through Draper-TRAF4-JNK signalling. Nat Commun 8:14355 (2017). Read more (PubMed: 28165006) »
See all 19 Publications for this product

Publishing research using ab6808? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

I am looking for a secondary antibody, a anti-mouse which is HRP conjugated. It has to be rat absorbed and suitable for Western blots. Can you help? Thankyou Rebecca Upton NHLI London

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Answer

We do not have such an antibody available at present, however we have sourced some and these will be added to our website catalogue by 25th May. You should be able to find them by browsing the secondary antibodies section under anti-mouse (choose from the list of conjugates to narrow the search). The product codes will be ab7061 and ab7068 (raised in donkey and goat, respectively).

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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