Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)

Submit a review Q&A (1)References (11)

Key features and details

  • Sheep Anti-Rabbit IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Sheep
  • Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr

Overview

  • Product name

    Sheep Anti-Rabbit IgG H&L (HRP)
    See all IgG secondary antibodies

  • Host species

    Sheep

  • Target species

    Rabbit

  • Tested applications

    Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details

  • Immunogen

    Rabbit IgG, whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Horseradish Peroxidase (HRP).

  • Conjugation notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads

  • Clonality

    Polyclonal

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/500 - 1/2500.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Images

  • IHC - Wholemount - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)
    IHC - Wholemount - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)This image is courtesy of an Abreview submitted by Michal Szlis

    See Abreview

  • Western blot - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)
    Western blot - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)
    Ab6795 was used at dilution 1/2000 with the primary antibody ab8895 in WB. See the review on ab8895.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (11)

Publishing research using ab6795? Please let us know so that we can cite the reference in this datasheet.

ab6795 has been referenced in 11 publications.

  • Hu J  et al. Effects of menstrual blood-derived stem cells on endometrial injury repair. Mol Med Rep 19:813-820 (2019). PubMed: 30569163
  • Zhao J  et al. Effects of paclitaxel intervention on pulmonary vascular remodeling in rats with pulmonary hypertension. Exp Ther Med 17:1163-1170 (2019). PubMed: 30679989
  • Yuan J  et al. MIR17HG-miR-18a/19a axis, regulated by interferon regulatory factor-1, promotes gastric cancer metastasis via Wnt/ß-catenin signalling. Cell Death Dis 10:454 (2019). PubMed: 31186404
  • Nasirzadeh M  et al. Crocetin promotes angiogenesis in human endothelial cells through PI3K-Akt-eNOS signaling pathway. EXCLI J 18:936-949 (2019). PubMed: 31762720
  • Jabarpour M  et al. Hyperbilirubinemia-induced pro-angiogenic activity of infantile endothelial progenitor cells. Microvasc Res 118:49-56 (2018). WB . PubMed: 29476756
View all Publications for this product

Customer reviews and Q&As

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Question

Hi Abcam,

I tried contacting the abcam distributer here in Australia (Sapphire bioscience), but the representative was unable to answer my questions.

I would like to purchase one secondary antibody against primary abcam antibodies I already have.

I would like to use the antibodies in a dot blot assay, using the ECL detection method.

There are three possible secondary antibodies that seem to me like they will work for two of my primaries:

Primary: ab8898 and ab9051
Secondary: ab97064, ab97095 and ab97200

Which secondary should I choose that will be best for both of my primaries, using ECL detection in a Dot Blot assay?

Thank you in advance for your assistance.

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Answer

Thank you for contacting us.

The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not tested them specifically for this application and thus cannot guarantee that it would work.

Therefore, I would like to recommend one of the following secondaries which have been tested in dot blot:

ab6721: https://www.abcam.com/index.html?datasheet=6721 (or use the following: https://www.abcam.com/index.html?datasheet=6721).

ab6802: https://www.abcam.com/index.html?datasheet=6802 (or use the following: https://www.abcam.com/index.html?datasheet=6802).

ab6795: https://www.abcam.com/index.html?datasheet=6795 (or use the following: https://www.abcam.com/index.html?datasheet=6795).

They are raised in goat, donkey and sheep and apart from this are equally well suited for your experimental setup. If you need a decision guiding you could for example choose the one with the most references: this would be ab6721 with 52 publications mentioning the use of this antibody.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (https://www.abcam.com/Abpromise).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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