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Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)

Submit a review Q&A (1)References (18)
IHC - Wholemount - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)
  • Western blot - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)

Key features and details

  • Sheep Anti-Rabbit IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Sheep
  • Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr

Conjugates logo Related conjugates and formulations

DyLight® 488 DyLight® 650 FITC Texas Red ® TRITC Unconjugated

Overview

  • Product name

    Sheep Anti-Rabbit IgG H&L (HRP)
    See all IgG secondary antibodies

  • Host species

    Sheep

  • Target species

    Rabbit

  • Tested applications

    Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details

  • Immunogen

    Rabbit IgG, whole molecule

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Horseradish Peroxidase (HRP).

  • Conjugation notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads

  • Clonality

    Polyclonal

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/2500.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC/IF
1/500 - 1/2500.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Images

  • IHC - Wholemount - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)
    IHC - Wholemount - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)This image is courtesy of an Abreview submitted by Michal Szlis
  • Western blot - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)
    Western blot - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)

    ab6795 was used at dilution 1/2000 with the primary antibody ab8895 in WB. See the review on ab8895.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (18)

Publishing research using ab6795? Please let us know so that we can cite the reference in this datasheet.

ab6795 has been referenced in 18 publications.

  • Afewerky HK  et al. Sodium-calcium exchanger isoform-3 targeted Withania somnifera (L.) Dunal therapeutic intervention ameliorates cognition in the 5xFAD mouse model of Alzheimer's disease. Sci Rep 12:1537 (2022). PubMed: 35087161
  • Jiang H  et al. CART mitigates oxidative stress and DNA damage in memory deficits of APP/PS1 mice via upregulating ß-amyloid metabolism-associated enzymes. Mol Med Rep 23:N/A (2021). PubMed: 33604684
  • Wirasinha RC  et al. Nfkb2 variants reveal a p100-degradation threshold that defines autoimmune susceptibility. J Exp Med 218:N/A (2021). PubMed: 33107914
  • Luo R  et al. Long non-coding RNA MEG3 inhibits apoptosis of retinal pigment epithelium cells induced by high glucose via the miR-93/Nrf2 axis. Am J Pathol N/A:N/A (2020). PubMed: 32473920
  • Liu T  et al. Circular RNA hsa_circRNA_002178 silencing retards breast cancer progression via microRNA-328-3p-mediated inhibition of COL1A1. J Cell Mol Med 24:2189-2201 (2020). PubMed: 31957232
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Hi Abcam,

I tried contacting the abcam distributer here in Australia (Sapphire bioscience), but the representative was unable to answer my questions.

I would like to purchase one secondary antibody against primary abcam antibodies I already have.

I would like to use the antibodies in a dot blot assay, using the ECL detection method.

There are three possible secondary antibodies that seem to me like they will work for two of my primaries:

Primary: ab8898 and ab9051
Secondary: ab97064, ab97095 and ab97200

Which secondary should I choose that will be best for both of my primaries, using ECL detection in a Dot Blot assay?

Thank you in advance for your assistance.

Read More
Answer

Thank you for contacting us.

The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not tested them specifically for this application and thus cannot guarantee that it would work.

Therefore, I would like to recommend one of the following secondaries which have been tested in dot blot:

ab6721: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-ab6721.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-HRP-ab6721.html).

ab6802: https://www.abcam.com/Donkey-Rabbit-IgG-HL-HRP-ab6802.html (or use the following: https://www.abcam.com/Donkey-Rabbit-IgG-HL-HRP-ab6802.html).

ab6795: https://www.abcam.com/Sheep-Rabbit-IgG-HL-HRP-ab6795.html (or use the following: https://www.abcam.com/Sheep-Rabbit-IgG-HL-HRP-ab6795.html).

They are raised in goat, donkey and sheep and apart from this are equally well suited for your experimental setup. If you need a decision guiding you could for example choose the one with the most references: this would be ab6721 with 52 publications mentioning the use of this antibody.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (https://www.abcam.com/Abpromise).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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