Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)

Submit a review Q&A (1)References (5)

Overview

  • Product name
    Sheep Anti-Rabbit IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species
    Sheep
  • Target species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
  • Immunogen

    Rabbit IgG, whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Horseradish Peroxidase (HRP).
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/500 - 1/2500.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Images

  • IHC - Wholemount - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)
    IHC - Wholemount - Sheep Anti-Rabbit IgG H&L (HRP) (ab6795)This image is courtesy of an Abreview submitted by Michal Szlis

    See Abreview

  • Western blot - Sheep polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6795)
    Western blot - Sheep polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6795)
    Ab6795 was used at dilution 1/2000 with the primary antibody ab8895 in WB. See the review on ab8895.

References

This product has been referenced in:
  • Jabarpour M  et al. Hyperbilirubinemia-induced pro-angiogenic activity of infantile endothelial progenitor cells. Microvasc Res 118:49-56 (2018). WB . Read more (PubMed: 29476756) »
  • Madgwick A  et al. Neural differentiation modulates the vertebrate brain specific splicing program. PLoS One 10:e0125998 (2015). WB . Read more (PubMed: 25993117) »
See all 5 Publications for this product

Publishing research using ab6795? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Hi Abcam,

I tried contacting the abcam distributer here in Australia (Sapphire bioscience), but the representative was unable to answer my questions.

I would like to purchase one secondary antibody against primary abcam antibodies I already have.

I would like to use the antibodies in a dot blot assay, using the ECL detection method.

There are three possible secondary antibodies that seem to me like they will work for two of my primaries:

Primary: ab8898 and ab9051
Secondary: ab97064, ab97095 and ab97200

Which secondary should I choose that will be best for both of my primaries, using ECL detection in a Dot Blot assay?

Thank you in advance for your assistance.

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Answer

Thank you for contacting us.

The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not tested them specifically for this application and thus cannot guarantee that it would work.

Therefore, I would like to recommend one of the following secondaries which have been tested in dot blot:

ab6721: https://www.abcam.com/index.html?datasheet=6721 (or use the following: https://www.abcam.com/index.html?datasheet=6721).

ab6802: https://www.abcam.com/index.html?datasheet=6802 (or use the following: https://www.abcam.com/index.html?datasheet=6802).

ab6795: https://www.abcam.com/index.html?datasheet=6795 (or use the following: https://www.abcam.com/index.html?datasheet=6795).

They are raised in goat, donkey and sheep and apart from this are equally well suited for your experimental setup. If you need a decision guiding you could for example choose the one with the most references: this would be ab6721 with 52 publications mentioning the use of this antibody.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (https://www.abcam.com/Abpromise).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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