Sheep Anti-Rabbit IgG H&L (HRP) (ab97095)
Key features and details
- Sheep Anti-Rabbit IgG H&L (HRP)
- Conjugation: HRP
- Host species: Sheep
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
-
Product name
Sheep Anti-Rabbit IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Sheep -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Conjugation
HRP
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
Conjugation notes
Molar enzyme: antibody protein ratio is 1:1 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
Our Abpromise guarantee covers the use of ab97095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC | Use at an assay dependent dilution. | |
IHC-P | 1/200 - 1/5000. | |
ELISA | 1/10000 - 1/100000. (Primary ELISA) | |
WB | 1/5000 - 1/50000. (Colorimetric: 1/5000-1/30000; Chemiluminescent: 1/10000-1/50000) |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (3)
ab97095 has been referenced in 3 publications.
- Cheng Q et al. SIX1 and DACH1 influence the proliferation and apoptosis of hepatocellular carcinoma through regulating p53. Cancer Biol Ther 19:381-390 (2018). PubMed: 29333942
- Chiu LLY et al. Comparisons of Auricular Cartilage Tissues from Different Species. Ann Otol Rhinol Laryngol 126:819-828 (2017). PubMed: 29078705
- Tang CK et al. The chemotherapeutic agent DMXAA as a unique IRF3-dependent type-2 vaccine adjuvant. PLoS One 8:e60038 (2013). ELISA . PubMed: 23555875