Sheep Anti-Rabbit IgG H&L (Texas Red ®) (ab6793)

Submit a review Q&A (3)References (11)

Key features and details

  • Sheep Anti-Rabbit IgG H&L (Texas Red ®)
  • Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
  • Host species: Sheep
  • Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IF

Overview

  • Product name

    Sheep Anti-Rabbit IgG H&L (Texas Red ®)
    See all IgG secondary antibodies

  • Host species

    Sheep

  • Target species

    Rabbit

  • Tested applications

    Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IFmore details

  • Immunogen

    Rabbit IgG, whole molecule

  • Conjugation

    Texas Red ®. Ex: 596nm, Em: 620nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.7 moles Texas Red per mole of Goat IgG

  • Conjugation notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose

  • Clonality

    Polyclonal

  • Research areas

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6793 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.
    ICC/IF
    1/1000 - 1/5000. PubMed: 21048978
    Notes
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.
    ICC/IF
    1/1000 - 1/5000. PubMed: 21048978

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (11)

    Publishing research using ab6793? Please let us know so that we can cite the reference in this datasheet.

    ab6793 has been referenced in 11 publications.

    • Hughes FM  et al. Bladder fibrosis during outlet obstruction is triggered through the NLRP3 inflammasome and the production of IL-1ß. Am J Physiol Renal Physiol 313:F603-F610 (2017). PubMed: 28592436
    • Liao CJ  et al. Lipocalin 2 induces the epithelial-mesenchymal transition in stressed endometrial epithelial cells: possible correlation with endometriosis development in a mouse model. Reproduction 147:179-87 (2014). PubMed: 24194573
    • Khan M  et al. Two-photon imaging of Zn2+ dynamics in mossy fiber boutons of adult hippocampal slices. Proc Natl Acad Sci U S A 111:6786-91 (2014). PubMed: 24757053
    • Gonzales AL  et al. A PLC?1-dependent, force-sensitive signaling network in the myogenic constriction of cerebral arteries. Sci Signal 7:ra49 (2014). PubMed: 24866019
    • Muxel SM  et al. NF-?B drives the synthesis of melatonin in RAW 264.7 macrophages by inducing the transcription of the arylalkylamine-N-acetyltransferase (AA-NAT) gene. PLoS One 7:e52010 (2012). ICC/IF . PubMed: 23284853
    View all Publications for this product

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    Customer is interested in using ab10554 in confocal microscopy. He's interested in using ab6717 and ab6793 as the secondary antibodies. How long approximately will the fluorescent signal last?

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    Answer

    Thank you for your enquiry. We cannot give you an exact time before which the signal will fade with these fluorophore conjugated secondary antibodies. The signal could last a week (the time frame that you mentioned that you would need) if your samples are prepared and stored under the correct conditions (at 4C in the dark). However, we do not know this for sure. Also, some fluorophores will fade faster than others. Please note that ab10554 has not yet been tested for application in confocal microscopy. If you have any additional questions, please contact us again.

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    Question

    GENERAL TECHNICAL ENQUIRY - Mon 01 Mar 2004, 11:59hrs ===================================================== ANTIBODY CODE ab6793 BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM i would like to ask about the isoelectric point of this anibody and its F(ab')2 fragments, if possible.

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    Answer

    Thank you for your enquiry. We are very sorry but we don't determine the pI.

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    Question

    is your polyclonal anti-rabbit Ab absorbed against rat and mouse serum?

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    Answer

    The immunogen is Rabbit IgG, whole molecule, so Ab6793 recognizes rabbit immunoglobulins specifically. We have no information if this antibody cross-reacts with rat and mouse IgG.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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