Sheep Anti-Rabbit IgG H&L (Texas Red ®) (ab6793)

Submit a review Q&A (3)References (9)

Overview

  • Product name
    Sheep Anti-Rabbit IgG H&L (Texas Red ®)
    See all IgG secondary antibodies
  • Host species
    Sheep
  • Target species
    Rabbit
  • Tested applications
    Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IFmore details
  • Immunogen

    Rabbit IgG, whole molecule

  • Conjugation
    Texas Red ®. Ex: 596nm, Em: 620nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.7 moles Texas Red per mole of Goat IgG
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.
ICC/IF 1/1000 - 1/5000. PubMed: 21048978

References

This product has been referenced in:
  • Khan M  et al. Two-photon imaging of Zn2+ dynamics in mossy fiber boutons of adult hippocampal slices. Proc Natl Acad Sci U S A 111:6786-91 (2014). Read more (PubMed: 24757053) »
  • Gonzales AL  et al. A PLC?1-dependent, force-sensitive signaling network in the myogenic constriction of cerebral arteries. Sci Signal 7:ra49 (2014). Read more (PubMed: 24866019) »
See all 9 Publications for this product

Publishing research using ab6793? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

Customer is interested in using ab10554 in confocal microscopy. He's interested in using ab6717 and ab6793 as the secondary antibodies. How long approximately will the fluorescent signal last?

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Answer

Thank you for your enquiry. We cannot give you an exact time before which the signal will fade with these fluorophore conjugated secondary antibodies. The signal could last a week (the time frame that you mentioned that you would need) if your samples are prepared and stored under the correct conditions (at 4C in the dark). However, we do not know this for sure. Also, some fluorophores will fade faster than others. Please note that ab10554 has not yet been tested for application in confocal microscopy. If you have any additional questions, please contact us again.

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Question

GENERAL TECHNICAL ENQUIRY - Mon 01 Mar 2004, 11:59hrs ===================================================== ANTIBODY CODE ab6793 BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM i would like to ask about the isoelectric point of this anibody and its F(ab')2 fragments, if possible.

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Answer

Thank you for your enquiry. We are very sorry but we don't determine the pI.

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Question

is your polyclonal anti-rabbit Ab absorbed against rat and mouse serum?

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Answer

The immunogen is Rabbit IgG, whole molecule, so Ab6793 recognizes rabbit immunoglobulins specifically. We have no information if this antibody cross-reacts with rat and mouse IgG.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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