Anti-Shiga Toxin A subunit antibody [1C6A6G9] (ab101839)

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Overview

  • Product name
    Anti-Shiga Toxin A subunit antibody [1C6A6G9]
  • Description
    Mouse monoclonal [1C6A6G9] to Shiga Toxin A subunit
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity

    Predicted to work with: Escherichia coli
  • Immunogen

    Synthetic peptide (

    REFRQALSETAPVYT

    ) corresponding to amino acids 198-212 of Shiga Toxin A subunit.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.09% Sodium azide
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    1C6A6G9
  • Isotype
    IgG1

Applications

Our Abpromise guarantee covers the use of ab101839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 36 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    Shiga toxins are a family of related toxins with two major groups, Stx1 and Stx2, whose genes are considered to be part of the genome of lambdoid prophages. The most common sources for Shiga toxin are the bacteria S. dysenteriae and the Shigatoxigenic group of Escherichia coli (STEC), which includes serotype O157:H7 and other enterohemorrhagic E. coli (EHEC).
  • Database links
    • Alternative names
      • Stx2 A subunit antibody

    References

    ab101839 has not yet been referenced specifically in any publications.

    Publishing research using ab101839? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

    10
    Votes: Highest First

    Are they using the purified protein or anEHEC O157:H7 lysate? If the later, I would suggest to increase the protein load up to 20 to 30ug per lane to offer the antibody enough target protein.
    - They used lysate and it's hard to get enough sample, ...

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    Thank you for your reply.


    Indeed, usually the blocking step is connected with the occurrence of side bands. However, changing the blocking agent can significantly increase the signal strength as our lab found out. I have attached a PDF...

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    LOT NUMBER gr37811-4 ORDER NUMBER 1013227 DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE EHEC O157:H7 PRIMARY ANTIBODY Concentration or dilution: 1/250-1/1000 Diluent buffer: 2% skim milk Incubation time: o/n (˜18 hrs) Incubation temperatur...

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    Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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